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Position: Home > Articles > Sequence Analysis of OmpH Gene of 8 Avian Pasteurella multocida Strains China Animal Husbandry & Veterinary Medicine 2013,40 (12) 43-47

8株禽多杀性巴氏杆菌外膜蛋白H基因序列分析

作  者:
高明燕;徐步;龚建森;王鑫;俞燕;范建华;刘学贤
单  位:
中国农业科学院家禽研究所
关键词:
禽多杀性巴氏杆菌;OmpH基因;序列分析;同源性
摘  要:
为了解国内禽多杀性巴氏杆菌(Pasteurella multocida,Pm)流行株外膜蛋白H(OmpH)基因的变异情况,参考GenBank中已发表的多杀性巴氏杆菌序列设计1对特异性引物,采用PCR方法对不同来源的8株禽Pm菌株和3个荚膜型参考株(A、B、D)的OmpH基因进行扩增、测序。结果显示,11个菌株的OmpH基因开放阅读框在1002~1071bp之间;SignalIP 4.0预测结果表明,信号肽均为N端20个氨基酸残基,成熟蛋白氨基酸残基数量在314~337aa之间,推测的分子质量在33.76~37.04ku之间。与GenBank中15个菌株OmpH基因序列比对结果发现,核苷酸同源性在84.9%~100.0%之间;氨基酸同源性在81.5%~100.0%之间;其中C48-1、1010、9003、890920、921012、XJ-e 6个国内禽Pm分离株OmpH序列同源性为100.0%。试验结果表明,国内禽Pm菌株OmpH基因非常保守。
译  名:
Sequence Analysis of OmpH Gene of 8 Avian Pasteurella multocida Strains
作  者:
GAO Ming-yan;XU Bu;GONG Jian-sen;WANG Xin;YU Yan;FAN Jian-hua;LIU Xue-xian;Poultry Institute,Chinese Academy of Agricultural Sciences;
关键词:
avian Pasteurella multocida;;OmpH gene;;sequence analysis;;homology
摘  要:
According to OmpH gene of Pasteurella multocida published in GenBank,apair of primers was designed.OmpH gene of 8avian Pasteurella multocida strains and three capsular-type reference strains(A,B,D)were amplified by PCR and sequenced.The results showed that the ORF of OmpH gene were from 1002to 1071bp.The predicted maturation proteins were composed of 314to 337amino acids except 20-amino-acid signal peptide.The molecular weights of maturation proteins were from 33.76to 37.04ku.The alignment data indicated that the nucleotide sequence homologies were 84.9%to100.0%,and the deduced amino acid homologies were 81.5%to 100.0%compared with 15reference OmpH sequence of Pasteurella multocida published in GenBank.The homomologies of OmpH sequences of six avain Pasteurella multocida strains isolated from China were 100.0%.The results revealed that the OmpH genes of avain Pasteurella multocida strains isolated from China were very conservative.

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