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Position: Home > Articles > Isolation and Selection of Probiotic Lactobacillus Stains from Traditional Fermented Sausages MEAT RESEARCH 2008 (12) 49-53

发酵香肠中益生性乳杆菌的分离及筛选

作  者:
马德功;王成忠;张志国;于功明;崔文文
单  位:
青岛明月海藻集团有限公司;山东轻工业学院
关键词:
发酵香肠;益生菌;乳杆菌;筛选
摘  要:
利用PH2.5和1%胆盐的MRS液体培养基,对自然发酵香肠中耐低酸耐胆盐的乳杆菌进行富集,从中分离纯化得到184株乳杆菌。通过一级筛选:24h产酸、耐盐耐硝性、产粘液、产过氧化氢等实验,筛选出20株符合发酵香肠乳酸菌发酵剂基本要求的菌株。通过二级筛选:耐低酸能力、耐胆汁酸盐能力和抑菌能力,最终筛选出了性能优良乳杆菌3株,可作为益生型发酵剂用于发酵香肠生产。文中还对所获得菌株的相关发酵性能进行了测定。
译  名:
Isolation and Selection of Probiotic Lactobacillus Stains from Traditional Fermented Sausages
作  者:
MA Degong1,2, WANG Chengzhong2, ZHANG Zhiguo2, YU Gongming2, CUI Wenwen1 (1. Qingdao Bright Moon Seaweed Co.,Ltd, Jiaonan 266400, China; 2. Shandong College of Food and Biology Engineering, Shandong Jinan 250353, China)
关键词:
fermented sausages; probiotics; lactobanillus; selection
摘  要:
A total of 184 lactobanillus stains was isolated and purified from naturally fermented dry sausages after enrichment in MRS broth at pH 2.5 followed by 1% bie salt stressing.After selected by first grade: 24h acid-generation, NaCl and nitrite tolerance, slime production,H2O2-producing and so on,20 strains that meet the fundmental requirements of fermented sausages starter cultures were ob- tained for second selection including:capability of endure low acid, capability of endure cattle gallladder salt and inhitory activity.Finally, three strains with excellent characteristies were obtained, and they can be used as probiotic starter cultures for fermented sausages production.The fermented characteristics of these three strains wre also studied in this paper.

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