单 位：
天津农学院农学与资源环境学院;天津农学院食品科学与生物工程学院
关键词：
三赞胶;多糖;降解菌;分子量;抗氧化活性
摘 要：
筛选得到3株三赞胶降解菌,可在以三赞胶为唯一碳源的培养基平板上产生降解圈,多项分类鉴定结果表明,降解菌JD05和JD08分别为荧光假单胞菌和巨大芽胞杆菌,降解菌SZJ01为烟曲霉。3株降解菌协同作用,37℃处理72 h,三赞胶溶液粘度下降79.6%,多糖分子量降低为2个不同分子量分布的组分,重均分子量分别为1.9×10~4 Da和3.7×10~3 Da,经生物降解处理后,三赞胶多糖降解产物的抗氧化活性提高,清除羟自由基和超氧阴离子的IC50分别为3.8 mg/mL和2.6 mg/mL。
译 名：
Screening, identification of sanxan-degrading microorganisms and antioxidant activities of degradation product
作 者：
CHENG Bin;LI Xiao-yan;PAN Yi-chen;FENG Xue;HUANG Hai-dong;College of Agronomy & Resources and Environment, Tianjin Agricultural University;College of Food Science and Bioengineering, Tianjin Agricultural University;
关键词：
sanxan;;polysaccharide;;degrading microorganisms;;molecular weight;;antioxidant activity
摘 要：
Sanxan-degrading strains were screened, which formed degrading band on medium plate with sanxan as the only carbon source. On the basis of polyphasic taxonomic study, strain JD05, JD08 and SZJ01 were considered to be Pseudomonas fluorescens, Bacillus megaterium and Aspergillus fumiga. Treated by the mixture fermentation liquid of these three strains with the temperature of 37 ℃ and 72 h, the viscosity of sanxan solution decreased by 79.6%. The molecular weight of polysaccharide was reduced to two components with different molecular weight distributions, with 1.9×10~4 Da and 3.7×10~3 Da, respectively. The antioxidant activity of sanxan degradation product increased, with the IC50 of scavenging hydroxyl free radical 3.8 mg/mL and the IC50 of scavenging superoxide anion 2.6 mg/mL, respectively.
