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Position: Home > Articles > Study on Extraction of β-carotene from Blakeslea trispora Journal of Anhui Agricultural Sciences 2014 (2) 253-254+288

三孢布拉氏霉中β-胡萝卜素的提取研究

作  者：
余茜炜;向梦雄;左乐;蔡俊;王常高
单  位：
湖北工业大学发酵工程教育部重点实验室工业发酵湖北省协同创新中心
关键词：
β-胡萝卜素;三孢布拉氏霉;提取
摘  要：
[目的]确定从三孢布拉氏霉干菌体中提取β-胡萝卜素的溶剂种类及提取条件。[方法]以发酵所得的三孢布拉氏霉干菌丝体为原料,通过单因素试验考察了不同溶剂种类、料液比、提取时间和提取温度对β-胡萝卜素提取得率的影响。[结果]试验得出,乙醇提取得率较低,而丙酮、石油醚、乙酸乙酯和正己烷4种溶剂从三孢布拉氏霉干菌体中提取β-胡萝卜素的得率几乎相同。从价格因素考虑,选取石油醚作为提取溶剂。适宜的提取条件为料液比1∶15 g/ml,提取时间2 h,提取温度40℃,提取得率可达约16 mgβ-胡萝卜素/g干菌体。[结论]该研究可为利用三孢布拉氏霉发酵法生产天然β-胡萝卜素提供依据和参考。
译  名：
Study on Extraction of β-carotene from Blakeslea trispora
作  者：
YU Xi-wei;Key Laboratory of Fermentation Engineering (Ministry of Education),Hubei Provincial Cooperative Innovation Center of Industrial Fermentation,Hubei University of Technology;
关键词：
β-carotene;;Blakeslea trispora;;Extraction
摘  要：
[Objective] To determine the extraction solvent and condition of β-carotene from Blakeslea trispora dry mycelium. [Method] Blakeslea trispora dry mycelium by fermentation was used as the material. The effects of solvent types,solid-liquid ratio,extraction time and extraction temperature on the yield of β-carotene were studied by single factor test. [Result]The extraction yield by ethanol is very low,but the yields by acetone,petroleum ether,ethyl acetate and n-hexane are almost the same. Petroleum ether was used as the final extraction solvent just because of its low cost. The optimum extraction conditions are 1∶ 15 g / ml of solid-liquid ratio,2 hours of extraction time and 40 ℃ of extraction temperature. The yield of extraction was up to 16 mg β-carotene per 1g dry mycelium. [Conclusion]The study could provide the basis and reference for the production of natural β-carotene by Blakeslea trispora fermentation.

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