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酵母原生质体制备、融合及高产β-胡萝卜素融合子的筛选

作  者:
王岁楼;王海翔;杨志萍;吕春晖;步芬
单  位:
中国药科大学药学院
关键词:
酵母;原生质体融合;β-胡萝卜素
摘  要:
为提高黏红酵母NR-98(生长温度28℃较高,但β-胡萝卜素产量较低)β-胡萝卜素发酵的产率,将其与法夫酵母Ph-1(β-胡萝卜素产量较高,但生长温度只有20℃)进行了原生质体融合.通过研究两种酵母菌原生质体制备、再生及融合的影响因素,最终筛选出了既能在28℃较高温度下生长、β-胡萝卜素产量又高于两出发菌株的具有遗传稳定性的融合子R-5,其β-胡萝卜素产量不仅比黏红酵母NR-98提高了53.33%,而且比法夫酵母Ph-1也提高了27.42%,达到9.20mg/L.
单  位:
College of Pharmacy,China Pharmaceutical University,Nanjing 210009,China
关键词:
yeasts%protoplast fusion%β-carotene
摘  要:
The protoplast fusion technique between R.glutinis NR-98 and Phaffia rhodozyma Ph-1 was studied.The strain NR-98 could grow at 28 ℃,but had a lower yield of β-carotene.In contrast,the strain Ph-1 had high yield of β-carotene,but could not grow at 28 ℃.The results of fusion experiments showed that the fusant R-5 revealed the parent strain properties of thermotolerance and β-carotene yield.When using glucose as substrate at 28 ℃,the fusant R-5 had a high β-carotene yield up to 9.20 mg/L,with the enhancement of 27.42% and 53.33% when compared with strain Ph-1 and strain NR-98,respectively.The genetic quality of fusant R-5 was good as confirmed by stability experiments.

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