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Position: Home > Articles > Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC FOOD SCIENCE 2007,28 (11) 404-407

反相高效液相色谱法测定笃斯越桔叶片中槲皮素含量

作  者:
张燕;林松毅;刘静波;王二雷;宋龙凤
单  位:
吉林大学军需科技学院营养与功能食品研究室
关键词:
笃斯越桔;槲皮素;RP-HPLC
摘  要:
采用ShimpackVP-ODS色谱柱(4.6mm×250mm,5μm),以甲醇-0.5%磷酸(50/50)为流动相,360nm为检测波长,用RP-HPLC法测定了笃斯越桔叶片中槲皮素的平均含量为0.78%;方法学验证结果为精密度实验RSD=1.13%(n=5),重现性实验RSD=1.73%(n=5),加样回收率平均值Y=101.6%,RSD=1.93%(n=5),说明本方法精密度、重现性、加样回收率均较好。
译  名:
Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC
作  者:
ZHANG Yan,LIN Song-yi,LIU Jing-bo,WANG Er-lei,SONG Long-feng (Laboratory of Nutrition and Functional Food, College of Quartermaster Technology, Jilin University, Changchun 130062, China)
关键词:
Vacciniumu Liginosum L.;quercetin;RP-HPLC
摘  要:
Determination of Quercetin in the leaves of Vacciniumu Liginosum L. was carried out. The main conditions of RP- HPLC were that: the column Shimpack VP-ODS(4.6mm×250mm, 5μm), mobile phase methanol and 0.5% of phosphoric acid (50/ 50), detecting wavelength 360 nm. The average content of quercetin was 0.78%. The results of methodology were: precision test RSD=1.13%(n=5), reproducibility test RSD=1.73%(n=5), average recovery rate of samples Y=101.6%, RSD=1.93% (n=5), which indicated that the precision, reproduction and recovery rate were proper for built of determination method.

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