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当前位置: 首页 > 文章 > 黄芪和板蓝根对猪细小病毒的体外抑制作用 西北农林科技大学学报(自然科学版) 2008 (10) 48-52+58
Position: Home > Articles > Study on inhibitive effect of Radix Astragali and Radix Isatidis in vitro on porcine parvovirus Journal of Northwest A & F University(Natural Science Edition) 2008 (10) 48-52+58

黄芪和板蓝根对猪细小病毒的体外抑制作用

作  者：
钟华;赵宝玉;范国英;刘俊伟;陈俊杰
单  位：
河南科技学院动物科学学院;西北农林科技大学
关键词：
黄芪;板蓝根;猪细小病毒(PPV)
摘  要：
【目的】探讨黄芪和板蓝根对猪细小病毒(PPV)的体外抑制作用。【方法】选用中草药黄芪、板蓝根作为抗病毒药物,利用细胞病变(CPE)抑制试验,测定黄芪与板蓝根单独使用及等体积合用,在PK-15单层细胞上对猪细小病毒的抑制作用。【结果】板蓝根单独使用时,体外试验对PPV有明显的抑制作用,对PPV的最小直接杀灭质量浓度为0.63 mg/mL,最小阻断质量浓度为0.31 mg/mL;黄芪单独使用时对PPV无明显的抑制作用;板蓝根与黄芪等体积合用时,对PPV的抑制作用显著增强,最小直接杀灭质量浓度提高为0.31 mg/mL,最小阻断质量浓度提高为0.038 mg/mL。【结论】黄芪与板蓝根联合使用抗病毒能力增强,且毒性较弱。
译  名：
Study on inhibitive effect of Radix Astragali and Radix Isatidis in vitro on porcine parvovirus
作  者：
ZHONG Hua1,2,ZHAO Bao-yu1,FAN Guo-ying2,LIU Jun-wei2,CHEN Jun-jie2 (1 College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China;2 College of Animal Science,Henan Insititute of Science and Technology,Xinxiang,Henan 453003,China)
关键词：
Radix Astragali;Radix Isatidis;porcine parvovirus(PPV)
摘  要：
【Objective】 The study was to investigate inhibitive effect on porcine parvovirus by Radix Astragali and Radix Isatidis in vitro.【Method】 Chinese herbs Radix Astragali and Radix Isatidis were adopted as antiviral drugs by utilizing the cytopthic effect(CPE) inhibitive experiment to measure the inhibitive effect on porcine parvovirus(PPV) by pig kidney(PK)-15 cell monolayer,when Radix Astragali and Radix Isatidis were used alone and association by equal volume.【Result】 Radix Isatidis has obviously inhibitive effect on experiment in vitro by individual using,the minimum concentration of direct kill was 0.63 mg/mL,the minimum concentration of blocking was 0.31 mg/mL;the Radix Astragali has not obviously inhibitive effection in experiment in vitro used alone.When Radix Astragali and Radix Isatidis were associated by one to one,the inhibitive effect on PPV has been enhanced obviously;the minimum concentration of direct killing was 0.31 mg/mL;and the minimum concentration of blocking was 0.038 mg/mL.【Conclusion】 The anti-virus capability were enhanced when Radix Astragali and Radix Isatidis were used in combination,at the same time toxicity were weakened.

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