单 位：
长江大学农学院昆虫研究所;中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
关键词：
白背飞虱;唾液腺;南方水稻黑条矮缩病毒;转录组;差异表达基因
摘 要：
【目的】白背飞虱Sogatella furcifera是南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)传播的主要介体,其唾液腺在取食和传毒的过程中都发挥着重要作用,本研究旨在通过对带毒与不带毒白背飞虱成虫的唾液腺进行转录组测序,进而比较分析找出差异表达基因,推测出唾液腺中与传毒相关的基因。【方法】利用Ion ProtonⅡ,PGM平台进行带毒与不带毒白背飞虱成虫唾液腺转录组测序,用SOAPdenovo软件从头组装,通过blast X比对进行基因注释。采用Blast2go和Blastall软件对所测基因的GO功能富集和KEGG代谢途径进行分析;釆用RPKM分析差异表达基因。【结果】带毒和不带毒白背飞虱的唾液腺转录组样本的原始数据经过软件处理后分别获得52 062和51 407条unigenes序列(GenBank登录号分别为SRS851833和SRS843978),平均长度分别为639和647 bp。通过NR数据库比对,共18 431个unigenes具有同源序列,与赤拟谷盗Tribolium castaneum的同源序列最多(16.23%)。所有unigenes富集到52个GO类群,240个KEGG信号通路。白背飞虱在感染南方水稻黑条矮缩病毒后,唾液腺有89个unigenes序列的表达发生变化。【结论】本研究通过比较分析带毒与不带毒白背飞虱唾液腺转录组样本后,发现有部分基因序列的表达存在差异,这些基因序列可能和病毒与载体的互作有关。转录组数据的获得为研究与取食和传毒相关的基因提供了有用的信息,从而在分子水平上为后续研究白背飞虱与SRBSDV互作机制奠定基础。
译 名：
Comparative transcriptome analysis of salivary glands of Southern rice black-streaked dwarf virus ( SRBSDV )-infected and uninfected adults of the white-backed planthopper, Sogatella furcifera ( Hemiptera:Delphacidae)
作 者：
DENG Yao;LIU Yu-Di;WANG Xiang-Ping;HOU Mao-Lin;Institute of Insect,College of Agriculture,Yangtze University;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;
关键词：
Sogatella furcifera;;salivary glands;;Southern rice black-streaked dwarf virus(SRBSDV);;transcriptome;;differentially expressed genes
摘 要：
【Aim 】The white-backed planthopper( WBPH),Sogatella furcifera,is a main vector of Southern rice black-streaked dwarf virus( SRBSDV). The salivary glands of the WBPH play an important role in feeding behaviour and virus transmission. This study aims to sequence the salivary glandtranscriptomes of viruliferous( SRBSDV-infected) and nonviruliferous adults of the WBPH,to find the differentially expressed genes in salivary glands and to further infer the virus-related genes. 【Methods】Salivary gland transcriptomes of viruliferous and nonviruliferous adults of S. furcifera were sequenced by using the Ion Proton II,PGM platform,and then were de novo assembled by SOAPdenovo software. Gene annotation was conducted by blast X,and GO term enrichment and KEGG metabolic pathway analysis were performed using Blast2 go and Blastall software. The differentially expressed genes were calculated by RPKM values. 【Results】A total of 52 062 unigenes from viruliferous salivary glands( VSGs) and51 407 unigenes from nonviruliferous salivary glands( NVSGs) of S. furcifera with the mean length of639 and 647 bp,respectively,were obtained,and their GenBank accession numbers are SRS851833 and SRS843978,respectively. A total of 18 431 unigenes have homologous sequences against the NR database,and the highest percentage of unigene sequences( 16. 23%) were matched to genes of Tribolium castaneum. All unigenes were enriched to 52 GO terms and 240 KEGG pathways. The results revealed that 89 unigenes had a significantly different expression level between VSGs and NVSGs.【Conclusion】Through the comparative analysis,the differentially expressed genes between viruliferous and nonviruliferous salivary glands of S. furcifera were found,and some of these genes might be involved in virus-vector interactions. The gene expression characteristics of salivary gland transcriptome provide useful information for the identification of genes involved in feeding and virus transmission and so lay the basis for investigating the interaction between SRBSDV and WBPH at the molecular level.
