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当前位置: 首页 > 文章 > 土庄绣线菊AMF多样性及季节变化分析 西部林业科学 2016,45 (4) 77-83+89
Position: Home > Articles > Diversity and Seasonal Variation of Arbuscular Mycorrhizal Fungi in Spiraea pubescens Journal of West China Forestry Science 2016,45 (4) 77-83+89

土庄绣线菊AMF多样性及季节变化分析

作  者：
朱艳育;峥嵘;段国珍;邵东华;王琚钢;白淑兰
单  位：
内蒙古农业大学;内蒙古师范大学
关键词：
土庄绣线菊;丛枝菌根真菌(AMF);季节变化;土壤因子
摘  要：
为探讨内蒙古大青山土庄绣线菊根围丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)多样性及季节变化规律,分别于2014年春、夏、秋3个季节采集土庄绣线菊根际土壤,采用湿筛倾析-蔗糖离心法分离AMF孢子,运用形态学方法进行种类鉴定。结果表明,AMF侵染强度、丛枝丰富度、孢子密度、种的丰富度的季节变化存在显著的差异,4个指标均显示夏季最高,秋季次之,春季最低。3个季节共分离出AMF10属44种,包括2个未知种,其中Rhizophagus、Funneliformis和Glomus为3个季节的共有优势属,Rhizophagus intraradices和Funneliformis mosseae为3个季节的共有优势种。丰富度、Shannon指数和Simpson指数与全N和速效P呈显著负相关,与速效K呈显著正相关。丰富度与p H值呈显著负相关,与碱解N呈显著正相关。土庄绣线菊根际AMF季节变化与植物物候一致,并与土壤因子密切相关。
译  名：
Diversity and Seasonal Variation of Arbuscular Mycorrhizal Fungi in Spiraea pubescens
作  者：
ZHU Yan-yu;ZHENG Rong;DUAN Guo-zhen;SHAO Dong-hua;WANG Ju-gang;BAI Shu-lan;College of forestry,Inner Mongolia Agricultural University;College of life science and technology,Inner Mongolia Normal University;
关键词：
Spiraea pubescens;;Arbuscular Mycorrhizal Fungi;;seasonal variation;;soil factors
摘  要：
In order to explore the arbuscular mycorrhizal fungi diversity and seasonal variation in rhizosphere of S. pubescens in Daqingshan Mountain,Inner Mongolia,China,rhizosphere soil samples were collected in spring,summer and autumn of 2014,respectively. AMF spores were separated with wet-sieving and decanting technique and identified by the morphological methods. The results indicated that the intensity of mycorrhizal colonization,the abundance of arbuscule,the spore density and the species abundance had significant differences between different seasons,and the maximum values of these four indexes were found in the summer,but the minimums were detected at spring. 44 AMF species belonging to 10 genera and 2 unidentified species were isolated from all soil samples,the dominant genera of 3 seasons were Rhizophagus, Funneliformis and Glomus. R. intraradices and F.mosseae were the dominant species of 3 seasons. Abundance,Shannon index and Simpson index had significantly negative correlation with total Nitrogen and available Phosphorus,while they had were significantly positive correlation with available potassium. Abundance had negatively correlated with p H,and it had significantly positively correlated with alkaline Nitrogen. The seasonal variation of AMF in rhizosphere of S. pubescens was consistent with plant phenology,and closely related to soil factors.

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