中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 白羽番鸭脂联素基因SNP与肌内脂肪和血清总胆固醇的关联分析 中国畜牧杂志 2011,47 (3) 6-10
Position: Home > Articles > SNP of Adiponectin Gene and Its Association with Intramuscular Fat and Serum Total Cholesterol in White Muscovy Chinese Journal of Animal Science 2011,47 (3) 6-10

白羽番鸭脂联素基因SNP与肌内脂肪和血清总胆固醇的关联分析

作  者：
张依裕;徐琪;段修军;张海波;赵文明
单  位：
扬州大学动物科学与技术学院;江苏畜牧兽医职业技术学院;贵州大学动物科学学院
关键词：
脂联素基因;SNP;关联分析;白羽番鸭
摘  要：
采用PCR-SSCP法对白羽番鸭脂联素基因全编码区和内含子进行多态性检测,并分析其多态对肌内脂肪(IMF)和血清总胆固醇(TC)的遗传效应,为番鸭分子标记辅助选择提供参考。结果表明:3个SNP分别在外显子1和外显子2的编码区发生A167G和G711A突变,为沉默突变,在内含子发生C290T突变;3个SNP位点均处于Hardy-Weinberg平衡状态(P>0.05),均表现为中度多态;关联分析表明,在A167G位点中,AA基因型个体的IMF和TC显著高于BB基因型个体(P<0.05);在C290T位点中,CC基因型个体的IMF显著高于TT基因型个体(P<0.05);其他各位点的各基因型与各性状间没有达到显著性差异(P>0.05)。该研究结果揭示,A167G和C290T位点对脂肪沉积可能有显著性的遗传效应,可作为番鸭的分子育种标记。
译  名：
SNP of Adiponectin Gene and Its Association with Intramuscular Fat and Serum Total Cholesterol in White Muscovy
作  者：
ZHANG Yi-yu1,2,XU Qi1,DUAN Xiu-jun3,ZHANG Hai-bo1, ZHAO Wen-ming1,SUN Guo-bo3,CHEN Guo-hong1(1.College of Animal Science and Technology,Yangzhou University,Jiangsu Yangzhou 225009,China; 2.College of Animal Science,Guizhou University,Guizhou Guiyang 550025,China; 3.Jiangsu Animal Husbandry & Veterinary College,Jiangsu Taizhou 225300,China)
关键词：
adiponectin gene;SNP;association analysis;White Muscovy
摘  要：
The whole coding region and intron of adiponectin gene were amplified with 4 pairs of primers designed from Cairina moschata DNA sequence(GQ873067) and its polymorphism was detected by PCR-SSCP method,and then its association with intramuscular fat(IMF) and serum total cholesterol(TC) were analysed.Three SNPs(A167G,T290C and G711A) were found.A167G and G711A,which belonged to silence mutations occurred in exon1 and exon2 coding region respectively,and T290C mutation was produced in intron.The three SNP loci presented medium polymorphism and were in accordance with Hardy-Weinberg equilibrium(P>0.05).Association analysis showed that the birds of genotype AA had significantly higher than genotype BB for IMF and TC in A167G locus(P<0.05).Then,the birds of genotype CC had significantly higher than genotype TT for IMF in T290C locus(P<0.05).The research suggested that the A167G and T290C loci had significant genetic effect on fatty deposition and may be used as markers of molecular breeding in White Muscovy.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46