Position: Home > Articles > Cloning of the Porcine adiponectin Promoter and Study on Genetic Polymorphism
Journal of Anhui Agricultural Sciences
2010,38
(1)
413-415+418
猪脂联素基因启动子克隆及多态性研究
作 者：
凌飞;汪亮亮;杜红丽;李仕新;陈瑶生
单 位：
华南理工大学生物与工程学院;华南农业大学动物科学学院;中山大学生命科学学院
关键词：
猪BAC文库;脂联素基因;启动子;SNP
摘 要：
[目的]为脂联素基因作为脂肪沉积候选基因的研究提供依据。[方法]通过猪BAC文库筛选及primer-walking的测序方法获得脂联素基因的启动子序列,采用PCR-RFLP技术对蓝塘猪、大花白猪、大白猪、长白猪和杜洛克猪5个品种共290头猪的脂联素基因启动子区的多态性进行了分析。[结果]脂联素基因的5’侧翼区-1 010 bp(G/A)的SNP位点,本地猪种中GG基因型频率明显高于外来猪种;-394 bp(T/C)的SNP位点,本地猪种基因型分布较为丰富,而在外来猪种中却没有检测到CC基因型,且外来猪中T等位基因频率较高。[结论]脂联素基因上游-1 010 bp(G/A)的SNP位点突变可能会引起该基因转录水平的改变,而-394 bp(T/C)的SNP位点与基因转录水平及与脂肪沉积可能无关。
译 名：
Cloning of the Porcine adiponectin Promoter and Study on Genetic Polymorphism
作 者：
LING Fei et al(School of Biology and Engineering,South China University of Technology,Guangzhou,Guangdong 510006)
关键词：
Porcine BAC library;Adiponectin;Promoter;SNP
摘 要：
[Objective] The aim was to provide basis for study on adiponectin as candidate gene of fat deposition.[Method] By screening porcine BAC library and using primer-walking method,the promoter sequence of adiponectin was obtained.The polymorphisms of 290 pigs were analyzed with PCR-RFLP technology,including Lantang pig,Large spotted pig,Large white pig,Landrace and Duroc.[Result] At SNP site of adiponectin 5'-flanking region-1 010 bp(G/A),GG genotype frequency in Chinese indigenous pigs was significantly higher than in exotic pigs;at SNP site of adiponectin 5'-flanking region-394 bp(T/C),the genotype distribution in Chinese indigenous pigs was abundant,while in exotic pig,CC genotype was not detected and T allele frequency was higher.[Conclusion] SNP site mutation of-1 010 bp(G/A)may lead to changes of the gene transcription level,while SNP site of-394 bp(T/C)may have no relation with gene transcription level and fat deposition.
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