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当前位置: 首页 > 文章 > 产毒黄曲霉颉颃菌的筛选及复合菌剂的构建 饲料工业 2022,43 (1) 59-64
Position: Home > Articles > Screening of Antagonistic Organism Against Toxin-Producing Aspergillus flavus and Preparation of Complex Strains Agents Feed Industry 2022,43 (1) 59-64

产毒黄曲霉颉颃菌的筛选及复合菌剂的构建

作  者：
黎欢;祁姣姣;彭玉莉;梁嘉旭;秦海啸;胡文锋
单  位：
华南农业大学食品学院;生物源生物技术(深圳)股份有限公司;广东省食品药品职业技术学校
关键词：
黄曲霉;不产毒霉菌;抑制作用;正交试验;复合菌剂
摘  要：
研究旨在获得能抑制产毒黄曲霉生长及产毒的高效能复合菌剂。用紫外荧光法和平板对峙法筛选出不产毒且能抑制产毒黄曲霉的颉颃菌株,应用形态学、分子生物学方法鉴定初筛菌株,通过牛津杯法测定初筛菌的发酵液对产毒黄曲霉及其初筛菌生长的影响,基于正交试验获得直观最佳抑制产毒黄曲霉生长及产毒的复合菌剂配方。结果表明:从土壤中筛选出的4株霉菌,分别鉴定为木霉(A-1、A-2)、曲霉(F-501)以及青霉(Q-281),对产毒黄曲霉生长的抑制率为木霉A-1 51%、木霉A-2 66%、曲霉F-501 61%、青霉Q-281 53%。A-1发酵液对产毒黄曲霉生长的抑制效果最明显,抑菌圈直径为18.00 mm。F-501、A-1发酵液分别对A-1、Q-281有抑制作用,抑菌圈直径分别为12.22、8.12 mm。A-2发酵液对A-1、F-501有抑制作用,抑菌圈直径均为10.52 mm。正交试验直观最优复合菌剂的配方:A-1、A-2、Q-281孢子浓度分别为1×10~4、1×10~6、1×10~6个/mL,其对黄曲霉毒素B1(AFB1)生成的抑制率为75%。分离得到的4株不产毒霉菌对产毒黄曲霉的生长抑制率均达到50%以上,复合菌剂对产毒黄曲霉的生长及产毒的抑制率为75%,可为从源头降解黄曲霉毒素提供候选菌株或复合菌剂。
译  名：
Screening of Antagonistic Organism Against Toxin-Producing Aspergillus flavus and Preparation of Complex Strains Agents
作  者：
LI Huan;QI Jiaojiao;PENG Yuli;LIANG Jiaxu;QIN Haixiao;HU Wenfeng;College of Food Science, South China Agricultural University;Guangdong Food And Drug Vocational-technical School;Bioforte Biotechnology (Shenzhen) Co., Ltd.;
关键词：
Aspergillus flavus;;nontoxigenic mold;;inhibition effect;;orthogonal test;;complex strains
摘  要：
This experiment was to obtain a high-effect complex strains which can inhibit the growth of toxin-producing Aspergillus flavus.The primary screening strains which were screened out by UV fluorescence method and plate confrontation method,were identified by the morphological characteristics and molecular biology method.The growth effect of the fermentation broth of the primary screen strains on toxin-producing Aspergillus flavus and the rest of the primary screen strains was determined by the Oxford cup method.Based on the orthogonal text,obtained the best complex strains formulation,which was able to inhibit the grown of toxin-producing Aspergillus flavus.The results showed that the best four strains were isolated and purified from soil samples,and among them,A-1 and A-2 were Trichoderma,F-501 was Aspergillus and Q-281 was Penicillium.The order of inhibition rate was A-1(51%),A-2(66%),F-501(61%),Q-281(53%).The fermentation broth of A-1 had the strongest inhibitory effect on the growth of toxin-producing Aspergillus flavus and the diameter of the inhibition zone was18.00 mm.The fermentation broth of F-501 and A-1 had inhibitory effect on A-1 and Q-281,respectively,and the diameter of the inhibition zone was 12.22 mm and 8.12 mm,respectively.The fermentation product of A-2 had inhibitory effect on A-1 and F-501,the diameter of the inhibition zone were both 10.52 mm.The optimal formula of complex strains was that,the spore concentration of A-1,A-2 and Q-281 were 1×10~4/mL,1×10~6/mL,and 1×10~6/mL,respectively,the inhibition rate of it to the aflatoxin B_1 was 75%.The growth inhibiton rate of the four primary screening strains and the complex strains to Aspergillus flavus was more than 50% and75%,respectively,which could provide candidate strains or complex strains for aflatoxin degradation from the source.

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