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Position: Home > Articles > Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS FOOD SCIENCE 2016,37 (14) 86-91

液相色谱-质谱法快速检测4种乳源低聚糖

作  者：
魏京华;陈历俊;赵军英;张毅;景萌娜;王品;姜铁民;乔为仓
单  位：
北京三元食品股份有限公司国家母婴乳品健康工程技术研究中心;大连工业大学生物工程学院
关键词：
超高效液相色谱-串联质谱;低聚糖;牛乳;人乳
摘  要：
建立一种乳样经简单预处理后采用超高效液相色谱-串联质谱同时检测4种重要的乳源低聚糖方法。牛乳及人乳经过脱脂、脱蛋白后经amide液相色谱柱用0.1%氨水-乙腈溶液梯度分离,采用质谱检测。2种唾液酸乳糖标准品的标准曲线在0.78~50.00 mg/L范围内线性良好(R~2>0.990),2种岩藻糖乳糖标准品的标准曲线在1.56~100.00 mg/L范围内线性良好(R~2>0.990)。加标回收率在80.00%~100.00%之间,重复性、精密度实验所得相对标准偏差均小于5.00%。
译  名：
Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS
作  者：
WEI Jinghua;CHEN Lijun;ZHAO Junying;ZHANG Yi;JING Mengna;WANG Pin;JIANG Tiemin;QIAO Weicang;School of Biological Engineering, Dalian Polytechnic University;National Maternal and Infant Health Dairy Researching Center of Beijing Sanyuan Foods Co.Ltd.;
关键词：
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS);;oligosaccharides;;bovine milk;;human milk
摘  要：
An ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was used to rapidly and simultaneously detect 4 oligosaccharides in bovine milk and human milk. Samples were defatted, deproteined, resolved on an ACQUITY BEH amide column using gradient elution with 0.1% ammonia water/acetonitrile as mobile phase, detected by mass spectrometric specific ion monitoring and quantified. The standard curves of 2 fucosyllactose oligosaccharides were linear within the range from 0.78 to 50.00 mg/L with R~2 > 0.990. The standard curves of 2 sialyllactose were linear within the range from 1.56 to 100.00 mg/L with R~2 > 0.990. The recoveries of spiked samples ranged from 80.00% to 100.00%. The relative standard deviation(RSD) of repeatability and precision was less than 5.00%.

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