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Position: Home > Articles > Somatotropic Efficacies of PCI-Ghrelin-28aa Carried with PLGA Microspheres China Animal Husbandry & Veterinary Medicine 2007,34 (7) 26-28

PCI-Ghrelin-28aa质粒微球对小鼠的促生长效应

作  者:
杨连玉;耿春银;谢金芳;刘佳佳;徐永平
单  位:
吉林农业大学动物科技学院;大连赛姆生物工程技术有限公司博士后科研流动站
关键词:
PLGA;Ghrelin;生长
摘  要:
以乳酸-乙醇酸共聚物为载体,采用复乳-液中蒸发法制备PCI-Ghrelin-28aa真核表达质粒微球,微球大小与文献报道相符,平均粒径分布在2~3μm间,测得的包封率为99.27%,载药量6.8μg/mg,回收率65.7%;将质粒微球肌肉注射小鼠,并记录25 d里小鼠的体重变化情况;体重统计结果表明,25 d时微球包裹质粒组累积增重比裸质粒组、生理盐水组分别高14.3%、27.9%(P<0.05)。结论认为,载PCI-Ghrelin-28aa质粒微球具有缓释作用,并有增强质粒吸收及表达的趋势。
译  名:
Somatotropic Efficacies of PCI-Ghrelin-28aa Carried with PLGA Microspheres
作  者:
YANG Lian-yu1,2,GENG Chun-yin1,XIE Jin-fang1,LIU Jia-jia1,XU Yong-ping2(1.Faculty of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2.Post-doctoral Research Center of Dalian SEM BIO-Engineering Technology Lt.d.Co,Dalian 116620,China)
关键词:
PLGA;Ghrelin;growth
摘  要:
Injectable sustained release PCI-Ghrelin-28aa were prepared by double emulsion-in liquid evaporation process,using biodegrable poly lactic acid as carrier.The result of transfection in vivo showed that after 25 days,accumulative increased body weights on the group injected with PCI-Ghrelin-28aa microspheres was higher than those group injected with naked plasmid(14.3%),saline(27.9%) respectively.In conclusion,PCI-Ghrelin-28aa microspheres have a controlled release effect and tendency to enhance absorb and expression of PCI-Ghrelin-28aa.

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