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Cre/lox系统介导的位点特异性重组技术及其应用

作  者:
李卫;郭光沁;郑国锠
单  位:
兰州大学细胞生物学研究所
关键词:
Cre/lox;定点整合;特定基因删除;诱变;基因克隆
摘  要:
 Cre/lox系统是源于P1噬菌体的一个DNA重组体系,它能导致在特定的DNA序列(loxP位点)处发生定点重组。该系统可以将外源基因定点整合到染色体上或将特定DNA片段删除;这种定位重组系统在遗传操作中发挥了重要的作用。
译  名:
Cre/lox System-mediated Site-specific Recombination Techniche and Its Application
作  者:
Le Wei Guo Guangqin Zheng Guochang Institute of Cell Biology,Lanzhou University,Lanzhou730000
关键词:
Cre/lox;site-specific integration;specifial gene deletion;mutagenesis;gene clone
摘  要:
Cre/lox system is a recombinase system which origins from bacteriophage P1.It can cause site-specific recombination at Loxp site.With this system,Site-specific integration or gene deletion at special gene or chromosome site can be obtained.It is also useful in mutagenesis and gene clone.

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