作 者：
冯德智;张利民;王际英;李宝山;马晶晶;蒋锦坤;张德瑞;姜柯君
单 位：
上海海洋大学水产与生命学院;山东省海洋水产研究所
关键词：
虹鳟;富马酸亚铁;血液生化指标;微量元素
摘 要：
本试验旨在研究富马酸亚铁对虹鳟幼鱼生长性能、血液生化指标及微量元素含量的影响。选择初始体重为(89.2±0.2)g的虹鳟幼鱼360尾,随机分成6组,每组3个重复,每个重复20尾鱼,分别饲喂添加富马酸亚铁0、20、40、80、160、480 mg/kg(以铁计)的试验饲料,各组分别为D0、D20、D40、D80、D160和D480组,D0组为对照组。各组铁实测含量分别为62.6、79.5、99.6、139.3、215.2、538.4 mg/kg,试验期60 d。结果表明:1)饲料中不同铁水平对虹鳟幼鱼生长性能无显著影响(P>0.05)。2)血红蛋白含量和红细胞数量呈现先上升后平稳的趋势;各组血细胞比容无显著差异(P>0.05)。3)各组全鱼、脊椎骨和肌肉铁含量随着饲料中铁水平的升高而升高,肝脏铁含量呈现先上升后平稳的趋势;各组全鱼锌含量无显著差异(P>0.05),D0组脊椎骨和肌肉锌含量显著高于其他各组(P<0.05),D0和D20组肝脏锌含量显著高于其他各组(P<0.05);全鱼铜含量随饲料铁含量的升高而升高,各组脊椎骨铜含量无显著差异(P>0.05),D0组肌肉铜含量显著低于其他各组(P<0.05)。4)D0组血清溶菌酶活性显著低于D20、D40、D80和D160组(P<0.05),但与D480组无显著差异(P>0.05);过氧化氢酶活性呈现先上升后下降趋势,D40组最高。由此可见,本试验条件下,以富马酸亚铁作为铁源,通过折线模型分析发现,以血红蛋白含量和肝脏铁含量为评价指标的虹鳟幼鱼对饲料中铁需要量分别为99.8和100.4 mg/kg。
译 名：
Effects of Ferrous Fumarate on Growth Performance,Blood Biochemical Parameters and Trace Element Contents of Juvenile Rainbow Trout (Oncorhynchus mykiss)
作 者：
FENG Dezhi1,2 ZHANG Limin2 WANG Jiying2 LI Baoshan2 MA Jingjing2 JIANG Jinkun1,2 ZHANG Derui1,2 JIANG Kejun1,2(1.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China; 2.Marine Fisheries Research Institute of Shandong Province,Yantai 264006,China)
关键词：
rainbow trout;ferrous fumarate;blood biochemical parameters;trace elements
摘 要：
This experiment was conducted to study the effects of ferrous fumarate on growth performance,blood biochemical parameters and trace element contents of juvenile rainbow trout(Oncorhynchus mykiss).A total of 360 fish with body weight of(89.2±0.2) g were randomly allotted to 6 groups with 3 replicates each and each replicate contained 360 fish.The fish were fed experimental diets supplemented with 0,20,40,80,160 and 480 mg/kg iron of ferrous fumarate(D0,D20,D40,D80,D160 and D480,D0 as a control group),respectively.The measured values of iron content in the six groups were 62.6,79.5,99.6,139.3,215.2,538.4 mg/kg,respectively.The experiment lasted for 60 days.The results showed as follows: 1) dietary iron level had no significant difference in growth performance among all groups(P>0.05).2) with increasing dietary iron level,haemoglobin and amount of red blood cells were increased firstly,and then leveled off,and no significant difference was found in haematocrit among all groups(P>0.05).3) Iron contents in whole body,vertebrae and muscle were increased with increasing dietary iron level,and liver iron content was increased firstly,and then leveled off from group D40 to D480.No significant difference was found in zinc content of whole body among all groups(P>0.05),zinc contents of vertebrae and muscle in group D0 were significantly higher than those in other groups(P<0.05),and liver zinc content in groups D0 and D20 was significantly higher than that in other groups(P<0.05).Copper content in whole body was increased with increasing dietary iron level,muscle copper content in group D0 was significantly lower than that in other groups(P<0.05),and no significant difference was observed in vertebrae copper content(P>0.05).4) Serum lysozyme activity in group D0 was significantly lower than that in groups D20,D40,D80 and D160(P<0.05),but had no significant difference in group D480(P>0.05);serum catalase activity appeared up-trend first and down-trend late,and group D40 was the highest.In conclusion,the dietary iron(as ferrous fumarate) requirements for juvenile rainbow trout estimated using the broken line model based on haemoglobin and iron content in liver are 99.8 and 100.4 mg/kg in the experiment.
