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Position: Home > Articles > Isolation,Purification and Anti-α-Glucosidase Activity of Polysaccharides from Mulberry Leaves FOOD SCIENCE 2011,32 (3) 112-116

桑叶多糖的分离纯化及对α-葡萄糖苷酶的抑制活性

作  者:
罗晶洁;王尉;曹学丽
单  位:
北京工商大学化学与环境工程学院北京市植物资源研究开发重点实验室
关键词:
桑叶;多糖;分离纯化;高效凝胶色谱;α-葡萄糖苷酶;抑制活性
摘  要:
利用Sephadex-G50从桑叶多糖中分离纯化得到两种不同多糖组分MLP1和MLP2,经过高效凝胶色谱(GPC)分析表明,MLP1的纯度为94.55%,重均相对分子质量MW为11800,多分散性系数为1.25,MLP2的纯度为96.64%,重均相对分子质量MW为7630,多分散性系数为1.12。并利用α-葡萄糖苷酶抑制活性体外筛选模型,对MLP1和MLP2进行活性研究,结果表明两个组分对α-葡萄糖苷酶均具有较好的抑制活性。
译  名:
Isolation,Purification and Anti-α-Glucosidase Activity of Polysaccharides from Mulberry Leaves
作  者:
LUO Jing-jie,WANG Wei,CAO Xue-li(Beijing Key Laboratory of Plant Resources Research and Development,College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China)
关键词:
mulberry leaves;polysaccharide;isolation and purification;gel permeation chromatographic (GPC);α-glucosidase;inhibitory activity
摘  要:
Two kinds of polysaccharides(MLP1 and MLP2) were isolated and purified from mulberry leaves by Sephadex-G50. The gel permeation chromatographic(GPC) analysis revealed that the purity,relative molecular weight and polydispersity of MLP1 was 94.55%,11800 and 1.25;Meanwhile,the purity,relative molecular weight and polydispersity of MLP2 was 96.64%,7630 and 1.12,respectively. Moreover,obvious inhibitory effects of MLP1 and MLP2 on α-glucosidase were observed.

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