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果梅离体繁殖技术研究

作  者:
谭志刚;陈红;张绿萍;程秀枝
单  位:
贵州省果树科学研究所;贵州大学
关键词:
果梅;腋芽;离体繁殖;生长调节剂
摘  要:
以优选果梅黔荔1号单株为材料,对外植体灭菌时间、腋芽启动和增殖培养进行了研究。结果表明:以0.1%HgCl_2消毒8 min效果最好,其存活率为75%;适合腋芽启动培养的基本培养基是WPM;最有利于腋芽启动和增殖的培养基是WPM+1.0 mg/L6-BA+0.5 mg/LNAA+1.0 mg/LGA_3,有效增殖系数高达1.51。
译  名:
A Preliminary Study of Prunus mume in Vitro Propagation
作  者:
TAN Zhi-gang~1,CHEN Hong~1,ZHANG Lv-ping~2,CHENG Xiu-zhi~1 (1.Research Institute for Fruit Resources of Karst Mountain Region of Guizhou University.Guiyang,Guizhou 550025;2.Pomology Research Institute of Guizhou,Guiyang Guizhou 550100)
关键词:
Prunus mume;;axillary bud;;in vitro propagation;;plant growth regulator
摘  要:
Using better single plant(Qian Li NO.1) from Prunus mume as materials,explants sterilization,and proliferation culture of axillary buds were studied.The results showed that the optimal sterilization time of explant was about 8 minutes with 0.1%HgCl_2,and its survival rate was 75%.The basal medium of of axillary buds was WPM.The best medium for and proliferation of axillary buds was WPM+1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA_3,and its effective multiplication coefficient was 1.51.

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