当前位置: 首页 > 文章 > 黄芪提取物及半合成产物的指纹图谱研究 海洋湖沼通报 2007 (1) 38-43
Position: Home > Articles > STUDIES ON THE CHROMATOGRAPHY FINGERPRINT OF THE RADIX ASTRAGALI EXTRACTION AND THE SEMI-SYNTHESIS PRODUCT Transactions of Oceanology and Limnology 2007 (1) 38-43

黄芪提取物及半合成产物的指纹图谱研究

作  者:
王惠勇;冯尚彩;程运丽
单  位:
临沂师范学院化学系;临沂市环境监测站
关键词:
指纹图谱;高效液相色谱;半合成产物;黄芪;黄芪甲苷
摘  要:
采用不同的提取方法和合成转化方法,建立了黄芪提取物及半合成产物的高效液相(HPLC)指纹图谱。黄芪提取物的HPLC检测条件为:Apollo-C18(150×4.6 mm,5μm)色谱柱,柱温40℃,流动相为乙腈:水(1:2),检测波长为205nm,流速为1.0 mL/min,进样量为10μl。结果表明:碱提法黄芪甲苷分离效果最好,由低到高的顺序为:水提法<醇提法<超声波法<碱提法;在2%硫酸溶液酸化条件下半合成产物的指纹图谱最好。建立的指纹图谱测定方法,适用于黄芪提取物及半合成产物的质量控制。
译  名:
STUDIES ON THE CHROMATOGRAPHY FINGERPRINT OF THE RADIX ASTRAGALI EXTRACTION AND THE SEMI-SYNTHESIS PRODUCT
作  者:
Wang Huiyoug1 FENG Shangcai2 CHENG Yun-li2(1.Environmental monitory center of Linyi,Linyi 276004,China;2.Department of chemistry,Linyi normal university,Linyi 276004,China)
关键词:
fingerprints;HPLC;semi-synthesis product;Radix astragali;astragaloside
摘  要:
Objective: The fingerprints of Radix astragali extraction and semi-synthesis product with different extracting and synthesis methods were established by high-performance liquid chromatography.Methods: The chromatographic condition: column: Apollo-C18(150 4.6 mm,5 μm),oven temperature: 40℃,mobile phase: Acetonitrile-water(33.3:66.7v/v),UV detection wavelength: 205 nm,flow rate: 1.0 mL min-1,injection volume: 10μl.Results: The alkali-extracting and semi-synthesis product treated with 2% sulfuric acid showed the best fingerprints.Conclusion: The fingerprints of Radix astragali and semi-synthesis product were established with high stability and good reproducibility,and this method could be used in quality control.

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