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Position: Home > Articles > Cloning and Expression of Chitinase Encoding Gene of Bacillus thuringiensis T04A001 Strain Hubei Agricultural Sciences 2011,50 (3) 177-180

苏云金芽孢杆菌几丁质酶基因的克隆及诱导表达

作  者:
蔡亚君;袁志明;胡晓敏;蔡全信
单  位:
中国科学院武汉病毒研究所;武汉纺织大学环境科学研究所
关键词:
苏云金芽孢杆菌;几丁质酶基因;克隆;序列分析;表达
摘  要:
从苏云金芽孢杆菌(Bacillus thuringiensis)T04A001中提取基因组DNA,通过PCR的方法克隆了几丁质酶chiAC基因(GenBank登录号为EF427670)。置换chiAC基因的启动子为T7启动子时,chiAC基因能在IPTG诱导下在大肠杆菌中大量表达,并产生大小约70 kDa的表达产物。
译  名:
Cloning and Expression of Chitinase Encoding Gene of Bacillus thuringiensis T04A001 Strain
作  者:
CAI Ya-jun1,2,YUAN Zhi-ming2,HU Xiao-min2,CAI Quan-xin2(1.Institute of Environmental Science,Wuhan Textile University,Wuhan 430073,China; 2.Wuhan Institute of Virology,The Chinese Academy of Sciences,Wuhan 430071,China)
关键词:
Bacillus thuringiensis;chitinase gene;clone;sequence analysis;expression
摘  要:
Chitinase gene chiAC(GenBank Accession Number:EF427670) was cloned by PCR from Bacillus thuringiensis T04A001 genomic DNA.Expression of chiAC under T7 promoter in Escherichia coli could induced by IPTG;and it produced a protein whose molecular weight was about 70 kDa.

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