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Position: Home > Articles > The Determination of Carotenoids in Apple Peel by HPLC Journal of Fruit Science 2001,18 (2) 34-36

高效液相色谱法测定苹果果皮的类胡萝卜素

作  者:
赵文恩;张劲强;乔宪生;俞宏;韩雅珊
单  位:
中国农业科学院郑州果树研究所;中国农业科学院郑州果树研究所!河南郑州450009郑州大学化工学院,河南郑州450052,中国农业科学院郑州果树研究所!河南郑州450009,中国农业科学院郑州果树研究所!河南郑州450009,中国农业科学院郑州果树研究所!河南郑州450009,中国农业大学食品学院!北京100094
关键词:
苹果果皮;类胡萝卜素;高效液相色谱法
摘  要:
用高效液相色谱法测定苹果果皮多种类胡萝卜素。以丙酮与石油醚提取色素 ,用NovapakC18(4 .6mm×15 0mm)色谱柱 ,乙腈∶甲醇∶二氯甲烷 (70∶2 0∶10 )流动相 ,在 45 0nm下检测类胡萝卜素。结果表明 ,玉米黄质、叶黄素、番茄红素与 β 胡萝卜素的回收率分别是94.2 7%~ 99.82 %、84.5 6 %~ 89.41%、87.5 3~ 93.5 4%与 88.0 8%~93 .2 4%。其中富士果仅检测到玉米黄质 (5 .9× 10 -3 μg/mm2 )与 β 胡萝卜素 (5 .4× 10 -3 μg/mm2 ) ;蛇果 (红星 )只有玉米黄质 (9.1× 10 -3 μg/mm2 )。
译  名:
The Determination of Carotenoids in Apple Peel by HPLC
作  者:
Zhao Wenen 1,2 ,Zhang Jinqiang 1,Qiao Xiansheng 1,Yu Hong 1,and Han Yashan 3 ( 1Zhengzhou Fruit Research Institute,CAAS,Zhengzhou 450009; 2Chemical Engineering College,Zhengzhou University,Zhengzhou 450052; 3College of Food Sciences,China Agri
关键词:
Apple peel;Carotenoids;High performance liquid chromatography
摘  要:
A reversed phase high performance liquid chromatographic method was used to determine carotenoids in apple peel.Carotenoids were extracted from apple peel with acetone and petroleum ether.Using Novapak C 18 column( 4.6 mm ×150 mm),acetonitrile∶methanol∶methylene chloride (70∶20∶10) as mobile phase,carotenoids were eluted and monitored at 450 nm.It was shown that recoveries of zeaxanthin,lutein,lycopene and β carotene were 94.27 %~ 99.82 %, 84.56 %~ 89.41 %, 87.53% ~ 93.54 %,and 88.08% ~ 93.24 %.‘Fuji’ apple contained zeaxanthin ( 5.9 ×10 -3 μg/mm 2) and β carotene ( 5.4 × 10 -3 μg/mm 2 ) and Red Delicious apple only zeaxanthin ( 9.1 ×10 -3 μg/mm 2).

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