关键词：
豇豆重花叶病毒;免疫捕获反转录;实时荧光PCR
摘 要：
为建立豇豆重花叶病毒(Cowpeasevere mosaic virus,CPSMV)免疫捕获-反转录-实时荧光(IC-RTreal-time)PCR分子检测方法,利用从ATCC和DSMZ引进的CPSMV标准毒株,先建立CPSMVRTreal-time PCR检测方法;在此基础上,建立用于CPSMV检测的IC-RTreal-time PCR方法.结果表明:IC-RTreal-time PCR方法的灵敏度可达500 pg叶组织;该方法具有特异性好、灵敏度高、操作简便等优点,省去了RNA的提取过程,适用于豇豆等豆类种子及种苗中对豇豆重花叶病毒的快速检测.
译 名：
Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus
作 者：
LI Bin,SU Han,WU Cui-ping,ZHOU Ming-hua,AN Yu-lin(Jiangsu Entry-Exit Inspection and Quarantine Bureau,Nanjing 210001,China
关键词：
Cowpea severe mosaic virus;immuno-capture reverse transcript;real-time PCR)
摘 要：
A method of immuno-capture reverse transcript real-time(IC-RT real-time)PCR assay was developed for detection of Cowpea severe mosaic virus(CPSMV).By introducing standard CPSMV strains from ATCC and DSMZ as materials,RT real-time PCR method was constituted firstly,which showed good amplification reaction and specificity.Based on the RT real-time PCR,IC-RT real-time PCR detection method for CPSMV was set up.The sensitivity of the assay could reach 500 pg leaf tissues.Meanwhile,the IC-RT real-time PCR assay was characteristic of good specificity,high sensitivity,convenient operation,and avoided RNA extraction,which was suitable for rapid detection of CPSMV from legume seeds and seedlings such as cowpea.
