作 者：
郭木金;廖富荣;陈青;林石明;陈红运;沈建国
单 位：
厦门出入境检验检疫局;福建出入境检验检疫局
关键词：
豇豆重花叶病毒;反转录-环介导等温扩增;检测
摘 要：
豇豆重花叶病毒(Cowpea severe mosaic virus,CPSMV)是我国进境植物检疫性有害生物,本研究根据CPSMV的外壳蛋白基因序列设计引物,以期建立CPSMV的反转录-环介导等温扩增(reverse transcription loop-mediated isothermal amplification,RT-LAMP)检测方法。条件优化后,建立了CPSMV的RT-LAMP方法,该方法可将CPSMV与同属其他病毒准确区分,具有良好的特异性。灵敏度试验显示,RT-LAMP比普通RT-PCR灵敏度高10倍。说明本研究建立的RT-LAMP方法适用于CPSMV的快速、特异和灵敏的检测。
译 名：
Detection of Cowpea severe mosaic virus by reverse transcription loop-mediated isothermal amplification
作 者：
Guo Mujin1,2,Liao Furong1,Chen Qing1,Lin Shiming1,Chen Hongyun1,Shen Jianguo3 (1.Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen 361026,China;2.Key Laboratory of Biopesticides and Chemical Biology,Fujian Agricultural and Forestry University,Ministry of Education,Fuzhou 350002,China;3.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350003,China)
关键词：
Cowpea severe mosaic virus;reverse transcription loop-mediated isothermal amplification;detection
摘 要：
Cowpea severe mosaic virus(CPSMV)is a quarantine pathogen in China.This study is aimed to establish a reverse transcription loop-mediated isothermal amplification(RT-LAMP) method for detection of CPSMV.The results showed that,under optimal reaction conditions,the RT-LAMP method could distinguish CPSMV from other comoviruses.A sensitivity test showed that this method was 10 times more sensitive than the standard RT-PCR assay.The method in this study is suitable for fast,specific and sensitive detection of CPSMV.
