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Position: Home > Articles > Study on the Tissue Culture and Propagation System of Gentiana scabra Bnnge Journal of Anhui Agricultural Sciences 2010,38 (12) 69-71

龙胆草组织培养及其无性系的研究

作  者:
文伟;杨骥
单  位:
辽宁铁岭师范高等专科学校
关键词:
龙胆草;组织培养;无性系
摘  要:
[目的]建立龙胆草组织苗繁育体系。[方法]以龙胆草的嫩茎为材料,进行愈伤组织的诱导与分化、不定芽的分化与生根、试管苗移栽与移植的研究。[结果]MS+0.2mg/LBA+1.0~1.5mg/L2,4-D是诱导嫩茎形成具有分化能力愈伤组织的理想培养基;MS+1.2mg/LAgNO3+0.6mg/LBA+0.1mg/LNAA是愈伤组织和不定芽分化培养的理想培养基;1/2MS+0.1mg/LIAA+0.3mg/LNAA是龙胆草试管苗生根培养和生根继代培养的理想培养基;移植到山坡上的试管苗保持了龙胆草的各项植物学性状。[结论]在该试验条件下,培育的试管苗移栽成活率达96%,说明此方法完全可以用于大田生产。
译  名:
Study on the Tissue Culture and Propagation System of Gentiana scabra Bnnge
作  者:
WEN Wei et al(Tieling Normal College of Liaoning Province,Tieling,Liaoning 112001)
关键词:
Gentiana scabra Bnnge;Tissue culture ;Clone
摘  要:
[Objective] The research aimed to establish the propagation system of tissue culture seedlings of Gentiana scabra Bnnge.[Method] Using the tender stems of G.scabra as materials,the callus induction and differentiation,the differentiation and rooting of adventitious buds,the transplanting of tube seedlings were studied.[Result] The ideal medium for inducing the tender stems to form the differentiation calli was MS+0.2 mg/L BA+1.0-1.5 mg/L 2,4-D.The ideal medium for the differentiation culture of calli and adventitious buds was MS+1.2 mg/L AgNO3+0.6 mg/L BA+0.1 mg/L NAA.The ideal medium for the rooting culture and rooting sub-culture of G.scabra tube seedlings was 1/2 MS+0.1 mg/L IAA+0.3 mg/L NAA.The botany characters of G.scabra tube seedlings transplanted on the hillside.[Conclusion] Under these experimental conditions,the transplanting survival ratio of cultured tube seedlings reached 96%,indicating that the method can be used in field production completely.

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