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Position: Home > Articles > Extraction,Purification and Antioxidantion of Polysaccharide from Purple Sweet Potato Acta Agriculturae Universitatis Jiangxiensis 2011,33 (4) 200-206

紫红薯多糖的提取纯化及抗氧化作用研究

作  者:
李翔;上官新晨;蒋艳;吴少福;陈继光
单  位:
江西农业大学食品科学与工程学院/江西省高校天然产物研究与开发重点实验室
关键词:
紫红薯;多糖;提取;纯化;抗氧化
摘  要:
实验优化了从紫红薯中提取多糖的工艺,利用DEAE-52柱层析分离纯化出紫红薯多糖Ⅰ和Ⅱ两个组分,并测定了对.OH、DPPH.和ABTS的清除作用。结果表明:紫红薯多糖最佳提取条件为提取时间60 min、料液比为1∶8、超声功率为240 W,经纯化后的紫红薯多糖Ⅰ和Ⅱ具有较好的清除自由基的能力。
译  名:
Extraction,Purification and Antioxidantion of Polysaccharide from Purple Sweet Potato
作  者:
LI Xiang,SHANGGUAN Xin-chen,JIANG Yan,WU Shao-fu,CHEN Ji-guang(College of Food Science and Engineering,The Key Laboratory for Research and Development of Natural Products,JAU,Nanchang 330045,China)
关键词:
purple sweet potato;polysaccharide;extraction;purification;antioxidant
摘  要:
The technology for extraction of polysaccharide from purple sweet potato was optimized by orthogonal array design.After removal of pigment and protein by X-5 and sevag method respectively,ⅠandⅡof polysaccharide portions were obtained by further purification by cellulose Chromatography DEAE.Additionally,the antioxidant capacity of the purified polysaccharide was evaluated by determining their DPPH·and·OH and ABTS scavenging activities.The results indicated that the obtained optimum conditions are material to water ratio 1∶8,extraction time 60 min,extraction power 240W.The yield of polysaccharide is 5.44% under the optimal conditions.After purification;Ⅰ and Ⅱof polysaccharide portions showed considerable antioxidant capacity.

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