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Position: Home > Articles > Molecular Identification of Five Wild-type Auricularia Strains Using Inter-simple Sequence Repeat (ISSR) Markers Acta Edulis Fungi 2008,15 (3) 7-12

五个野生木耳属菌株的ISSR分析

作  者:
贾定洪;郑林用;王波;郭勇;甘炳成;黄忠乾;彭卫红
单  位:
四川省农业科学院土壤肥料研究所
关键词:
木耳属;ISSR;遗传鉴定
摘  要:
以7个栽培菌株为参照,采用ISSR分子标记技术对5个野生木耳属菌株进行分析,使用NTSYSpc2.1生物软件对12个供试菌株进行聚类分析,构建系统树。试验筛选出了11个扩增谱带清晰、多态性好的ISSR引物,共扩增出78条清晰易辨的多态性谱带。聚类分析结果表明,利用ISSR技术可将全部供试材料区分开,遗传相似系数变异范围为0.108~0.822,在相似系数为0.51时,12个菌株聚为6个群,其中5个野生菌株各自聚为不同类群,遗传差异较大。
译  名:
Molecular Identification of Five Wild-type Auricularia Strains Using Inter-simple Sequence Repeat (ISSR) Markers
作  者:
JIA Dinghong,ZHENG Linyong,WANG Bo,GUO Yong,GAN Bingcheng,HUANG Zhongqian,PENG Weihong Soil and Fertilizer Institute,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066,China
关键词:
Auricularia;inter-simple sequence repeats;genetic identification
摘  要:
Five wild-type Auricularia strains were identified by ISSR(inter-simple sequence repeat)methodology using seven cultivated Auricularia strains as controls.Eleven ISSR primers,selected from a total of 60 test ISSR primers,amplified 78 distinct polymorphic DNA bands from the 12 Auricularia strains.ISSR similarity coefficients ranged from 0.108 to 0.822,and a dendrogram based on UPGMA(unweighted pair group method with arithmetic average) cluster analysis using these values revealed that the 12 strains were clustered into six groups(similarity coefficient 0.51).The five wild-type strains clustered into five different groups,and the most distinct strain genetically was the wild-type strain,Ausm(similarity coefficient 0.198).

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