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优良八仙花品种(Hydrangea serrata‘Preziosa’)的离体培养与快速繁殖

作  者:
殷丽青;胡永红;汤桂钧;黄卫昌;李世忠;王新其
单  位:
上海市农业科学院林木果树研究所;上海植物园;上海市闵行区农业科学研究所
关键词:
八仙花;组织培养;快速繁殖;观赏灌木
摘  要:
以八仙花当年生茎段为外植体,进行离体快速繁殖技术研究。结果表明:无菌芽诱导的适宜培养基为改良MS+BA 1.0 mg/L+NAA 0.1 mg/L+CH 0.5 g/L+蔗糖30 g/L+琼脂粉6.0 g/L,诱导率为97.2%;芽增殖的适宜培养基为改良MS+BA 1.0 mg/L+蔗糖30 g/L+琼脂粉6.0 g/L,增殖系数达6.1;生根的适宜培养基为1/2 MS+IBA 0.3 mg/L+蔗糖20 g/L+琼脂粉6.0 g/L,试管苗在该培养基上根系生长良好,生根率达100%。
译  名:
In vitro culture and rapid propagation of Hydrangea serrata 'Preziosa'
作  者:
YIN Li-qing1,HU Yong-hong2,TANG Gui-jun3,HUANG Wei-chang2,LI Shi-zhong3,WANG Xin-qi1(1Forestry and Pomology Research Institute,SAAS,Shanghai 201106,China;2Shanghai Botanical Garden,Shanghai 200231,China;3Agricultural Research Institute of Minghang District,Shanghai 201109,China)
关键词:
Hydrangea serrata 'Preziosa';Tissue culture;Rapid propagation;Ornamental shrub
摘  要:
Current stem segments of Hydrangea serrata 'Preziosa' were used as explants and rapid in vitro propagation of Hydrangea serrata 'Preziosa' were studied.The results showed that the medium suitable for inducion of the germ-free buds was the improved MS+1.0 mg/L BA+0.1 mg/L NAA+ 0.5 g/L CH+30 g/L sugar+6.0 g/L agar,with the inductivity of buds being 97.2%;the medium suitable for multiplication of the clumpy buds was the improved MS+1.0 mg/L BA+30 g/L sugar+6.0 g/L agar,with the multiplication rate being 6.1;and the medium suitable for rooting was 1/2 MS+0.3 mg/L IBA+20 g/L sugar+6.5 g/L agar,with the test-tube plantlets reaching 100% in rooting.

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