中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 烟草育苗基质典型腐生真菌的分离鉴定及其碳源代谢特征分析 中国烟草学报 2019 (1) 122-128
Position: Home > Articles > Isolation, identification, and carbon metabolism analysis of typical saprophytic fungi in tobacco seedling growing substrate Acta Tabacaria Sinica 2019 (1) 122-128

烟草育苗基质典型腐生真菌的分离鉴定及其碳源代谢特征分析

作  者：
周浩;向立刚;陈乾丽;汪汉成;余知和
单  位：
贵州省烟草科学研究院;长江大学生命科学学院
关键词：
育苗基质;腐生真菌;分离;鉴定;碳源代谢
摘  要：
【目的】烤烟育苗基质在育苗期易出现腐生真菌生长,本研究旨在了解腐生真菌的种类及其碳源代谢特征。【方法】采用组织分离培养法对腐生真菌进行分离,采用形态学和rDNA-ITS分析的方法对其进行鉴定,并利用Biolog代谢对其碳源代谢特征进行分析。【结果】共分离纯化获得3株腐生真菌,经鉴定确定菌株JZ-1和JZ-2为Trichoderma harzianum,菌株JZ-3为Trichoderma asperellum。这些腐生真菌创伤接菌时均可使烟苗发病,基质接菌时均不能使烟苗发病。它们能利用Biolog FF代谢板上44种碳源,其中赤藓糖醇、L-海藻糖、乳果糖、麦芽三糖、D-甘露糖、D-松三糖和D-山梨醇能促进其产孢。【结论】"木霉属真菌"是育苗基质上的腐生菌之一,具有较强的碳源代谢能力,研究结果对烟草育苗基质腐生真菌的认识奠定了基础。
译  名：
Isolation, identification, and carbon metabolism analysis of typical saprophytic fungi in tobacco seedling growing substrate
作  者：
ZHOU Hao;XIANG Ligang;CHEN Qianli;WANG Hancheng;YU Zhihe;College of Life Science, Yangtze University;Guizhou Academy of Tobacco Sciences;
单  位：
ZHOU Hao%XIANG Ligang%CHEN Qianli%WANG Hancheng%YU Zhihe%College of Life Science, Yangtze University%Guizhou Academy of Tobacco Sciences
关键词：
seedling substrate;;saprophytic fungi;;isolation;;identification;;carbon source metabolism
摘  要：
Flue-cured tobacco seedling substrate is prone to saprophytic fungi growth. The purpose of this study is to understand the species of saprophytic fungi and their carbon source metabolism. Saprophytic fungi were isolated by tissue culture method and identi?ed by morphology and rDNA-ITS analysis. Metabolism analysis on Biolog FF metabolic plates was performed to analyze carbon source metabolism characteristics of the pathogens. Three strains of saprophytic fungi were isolated and puri?ed. Strains JZ-1 and JZ-2 were identi?ed as Trichoderma harzianum, strain JZ-3 was Trichoderma asperellum. Saprophytic fungi can infect tobacco seedlings by wounded inoculation method on tobacco leaves, while not by inoculation directly on seedling substrate. These fungi metabolized the 44 carbon sources on Biolog FF metabolic plate, among which, erythritol, L-trehalose, lactulose, maltotriose, D-mannose, D-melezitose, and D-sorbitol can promote its conidia production. It is concluded that Trichoderma is one of the saprophytic fungi existing on tobacco seedling substrate,which has strong carbon source metabolism ability. Results of this study may help to enhance our understanding of saprophytic fungi on tobacco seedling substrate.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46