作 者：
周继术;TORSTENSEN Bente Elisabeth;STUBHAUG Ingunn;吉红
单 位：
西北农林科技大学动物科技学院;挪威国家海产品营养研究所
关键词：
大豆油;鱼油;大西洋鲑;肝细胞;脂肪酸代谢
摘 要：
为探讨大豆油替代鱼油对大西洋鲑(Salmo salar L.)肝细胞脂肪酸代谢的影响,本试验分别以鱼油和大豆油为脂肪源配制2种试验饲料,并分别饲喂大西洋鲑幼鲑5个月。饲养结束后,分离和培养大西洋鲑肝细胞,一方面将细胞与碳14标记油酸([1-14C]OA)共孵育,然后测定细胞和细胞内各脂质成分以及脂肪酸β-氧化产物的放射活性;另一方面提取肝细胞总RNA,采用实时荧光定量聚合酶链式反应(RT-qPCR)测定脂肪酸结合蛋白3(FABP3)、脂肪酸结合蛋白10(FABP10)、Δ5去饱和酶(D5D)、Δ6去饱和酶(D6D)及肉碱棕榈酰转移酶Ⅰ(CPT-Ⅰ)基因的相对表达量。结果表明:1)鱼油组与大豆油组肝细胞内游离脂肪酸(FFA)放射活性百分率分别为(62.7±13.0)%和(55.9±9.1)%,2组间无显著差异(P>0.05);鱼油组与大豆油组肝细胞内磷脂(PLs)放射活性百分率分别为(20.9±8.7)%和(17.9±5.4)%,2组间无显著差异(P>0.05);鱼油组肝细胞内三酰甘油(TAG)放射活性百分率为(2.0±0.9)%,显著高于大豆油组的(0.8±0.4)%(P<0.05);鱼油组与大豆油组肝细胞内β-氧化产物放射活性百分率分别为(1.0±0.3)%和(1.0±0.2)%,2组间无显著差异(P>0.05)。2)大豆油组肝细胞中FABP3、D6D、D5D基因的相对表达量均显著高于鱼油组(P<0.05),而FABP10和CPT-Ⅰ基因的相对表达量在2组间无显著差异(P>0.05)。由此可见,大豆油替代饲料中的鱼油对大西洋鲑肝细胞内FFA、PLs合成及脂肪酸β-氧化等脂肪酸代谢途径无显著影响,但显著降低了肝细胞内TAG的合成,促进了肝细胞内脂肪酸的去饱和,因此大豆油替代鱼油在一定程度上影响了肝细胞内脂肪酸代谢。
译 名：
Effects of Replacing Dietary Fish Oil with Soybean Oil on Fatty Acid Metabolism in Hepatocytes of Atlantic Salmon(Salmo salar L.)
作 者：
ZHOU Jishu;TORSTENSEN Bente Elisabeth;STUBHAUG Ingunn;JI Hong;College of Animal Science and Technology,Northwest A& F University;National Institute of Nutrition and Seafood Research(NIFES);Skretting Aquaculture Research Centre(ARC);
关键词：
soybean oil;;fish oil;;Atlantic salmon;;hepatocytes;;fatty acid metabolism
摘 要：
In order to investigate the effects of replacing dietary fish oil(FO) with soybean oil on fatty acid metabolism in hepatocytes of Atlantic salmon(Salmo salar L.),a total of 50 post-smolt Atlantic salmon with an average body weight of(165 ± 15) g were fed diets containing either FO or soybean oil(SO) as lipid sources for 5 months,and then hepatocytes were isolated and cultured. Some of the cultured hepatocytes were incubated with [1-14C]oleic acid(OA) and then the radioactivity of the cell,cellular lipid component and fatty acid β-oxidation products were analysed. M eanwhile,total RNA of the other hepatocytes were harvested and the relative mRNA expression levels of fatty acid binding protein 3(FABP3),fatty acid binding protein 10(FABP10),delta 5 desaturase(D5D),delta 6 desaturase(D6D),and carnitine palmityl transferaseⅠ(CPT-Ⅰ) were determined by real-time quantitative polymerase chain reaction(RT-qPCR). The results show ed as follows: 1) the radioactive activity percentage(RAP) of free fatty acid(FFA) in hepatocytes of FO and SO groups was(62.7 ±13)% and(55.9 ±9.1)%,respectively,and was not significantly different(P >0.05); the RAP of phospholipids(PLs) in hepatocytes of FO and SO groups was(20.9 ±8.7)% and(17.9 ±5.4)%,respectively,and there was still no significant difference between them(P > 0.05); the RAP of triacylglycerol(TAG) in hepatocytes of FO group was(2.0 ± 0.9) %,which was significantly higher than(0.8 ± 0.4) % of SO group(P < 0.05); the RAP ofβ-oxidation products in hepatocytes of FO and SO groups was(1.0 ±0.3)% and(1.0 ±0.2)%,respectively,and there was no significant difference betw een them(P > 0.05). 2) The relative expression levels of FABP3,D5D,D6D genes of SO group were significantly higher than those of FO group(P < 0.05),while the relative expression levels of FABP10 and CPT-Ⅰ genes were not significantly different betw een FO and SO groups(P >0.05). The results indicate that replacing dietary FO with SO has no effects on fatty acid metabolism pathw ays,such as the synthesis of FFA and PLs,andβ-oxidation of fatty acid in hepatocytes,but can significantly decrease the synthesis of TAG and promote the fatty acid desaturation in hepatocytes. Therefore,the fatty acid metabolism in hepatocytes is affected in a certain by replacing dietary FO with SO.
