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当前位置: 首页 > 文章 > 一株野生食用菌的形态及分子鉴定 北方园艺 2017 (21) 159-162
Position: Home > Articles > Identification of a Wild Mushroom Based on Its Morphology and ITS Sequence Northern Horticulture 2017 (21) 159-162

一株野生食用菌的形态及分子鉴定

作  者：
王锋尖;周向宇;甘露
单  位：
汉江师范学院生物化学与环境工程系
关键词：
野生菌;ITS序列;形态学鉴定;湖北新记录种;赛武当自然保护区
摘  要：
以采自湖北赛武当自然保护区内的一株野生食用菌的子实体为试材,研究了其生态环境、形态结构,并从形态学角度对其进行了鉴定;对其ITS序列进行了扩增、测序,并在基因组数据库中比对确定其亲缘关系。结果表明:该野生菌为金盖褐环柄菇(Phaeolepiota aurea),属湖北省新记录种。该研究结果为进一步开发其应用价值奠定了基础。
译  名：
Identification of a Wild Mushroom Based on Its Morphology and ITS Sequence
作  者：
WANG Fengjian;ZHOU Xiangyu;GAN Lu;Department of Biochemistry and Environment Engineering,Hanjiang Normal University;
单  位：
Department of Biochemistry and Environment Engineering,Hanjiang Normal University
关键词：
wild mushroom;;ITS sequence;;identification of morphology;;newly recorded species of Hubei;;Saiwudang Nature Reserve
摘  要：
The ecological environment and the morphological structure of a wild mushroom picked from Saiwudang Nature Reserve were studied in this experiment.Meanwhile,the species was identified based on its morphological characteristics. Molecular identification was done by extracting its DNA,amplifying and sequencing its ITS sequence,and comparing the result with Gen Bank to determine the genetic relationship.The results showed that the wild strain was identified finally as the Phaeolepiota aurea,a new record species in Hubei Province.The result laid a foundation for the further development of its application value.

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