作 者：
蔡忠良;张静静;王淏冉;任欣欣;朱超杰;刘莎莎;崔文姗;冯军厂
单 位：
河南师范大学水产学院河南省水产动物养殖工程技术研究中心水产动物疾病控制河南省工程实验室
关键词：
鲤;乳酸菌;头肾细胞;不同处理方式;免疫应答
摘 要：
为探究乳酸乳球菌Z-2(Lactococcus lactis Z-2)不同处理方式对鲤的益生作用,试验共设置L. lactis Z-2菌株6种不同处理[活菌及上清液的混合物(mixture of live cells and supernatant,LCS)、无细菌培养上清液(cell-free culture supernatant,CS)、活菌(live cells,LC)、菌热灭活(heat-killed cells,HK)、菌超声破碎(ultrasonic broken of cells,UB)和胞外多糖(exopolysaccharides of CS, EPS-2)]与鲤[体重(47.66±0.43)g]头肾单核细胞体外共培养,检测其对鲤头肾单核细胞的增殖能力和吞噬活性,及孵育液中一氧化氮(NO)和免疫相关细胞因子表达量的影响。结果表明:LCS、LC、UB和EPS-2处理组较之对照组,均能显著增强头肾细胞的增殖能力和吞噬活性,HK处理组仅能显著增强头肾细胞的吞噬活性(P<0.05),其中LC(1.580±0.032)和LCS(1.520±0.058)分别对鲤头肾细胞增殖能力和吞噬活性具有最积极的促进作用。除CS组外,其余处理组均能显著提高孵育液中NO的合成量(P<0.05),EPS-2[(1.960±0.043)μmol/L]效果最为显著。所有处理组均能显著增加头肾细胞中细胞因子TNF-α、IL-1β、IL-6和TGF-β的合成量(P<0.05),并且分别是LCS[(23.11±1.31)pg/mL]、HK[(991.82±55.86)pg/mL]、LCS[(147.98±8.25)pg/mL]/CS[(147.38±7.94)pg/mL]和LCS[(139.56±9.69)pg/mL]处理组具有最佳的促进效果;而仅有LCS、CS和EPS-2处理组能显著提升IL-10的合成量(P<0.05),且LCS[(81.82±3.03)pg/mL]具有最佳的促进效果。研究表明,L. lactis Z-2的不同处理方式均对鲤表现出一定的免疫调节作用。
译 名：
Probiotic Characteristics of Different Preparations for Lactococcus lactis Z-2 on Cyprinus carpio L. in Vitro
作 者：
CAI Zhongliang;ZHANG Jingjing;WANG Haoran;REN Xinxin;ZHU Caojie;LIU Shasha;CUI Wenshan;FENG Junchang;College of Fisheries, Henan Normal University,Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Engineering Lab of Henan Province for Aquatic Animal Disease Control;
关键词：
Cyprinus carpio L.;;Lactococcus lactis;;head kidney cell;;different treatment method;;immune response
摘 要：
To investigate the effects of the oral administration of different treatments for(mixture of live cells and supernatant, LCS; cell-free culture supernatant, CS; live cells, LC; heat-killed cells, HK, ultrasonic broken of cells, UB; exopolysaccharides of CS, EPS-2) L. lactis Z-2 on the probiotic performance in head kidney cells of Cyprinus carpio [(47.66±0.43) g], these different treatments of L. lactis Z-2 were co-cultured with the head kidney cells of the Cyprinus carpio. The proliferation and phagocytosis activition of nitric oxide(NO) and cytokines erpressionin the culture medium were determined. The re-sults showed that the preparation methed LCS,LC, UB and EPS-2 of L. lactis Z-2 significantlyenhanced the proliferation and phagocytic activity of the head kidney cells, HK treatment group only significantly enhanced the phagocytic activity of thehead kidney cells(P<0.05), LC(1.580±0.032) and LCS(1.520±0.058) had the most positive effect on theproliferation and phagocytic activity of head kidney cells, respectively. Except for CS group, the other treat-ment groups significantly increased the amount of NO synthesis in the incubation solution(P<0.05), the NOsynthesis effect of the EPS-2 [(1.960±0.043) μmol/L] was the most significant. The synthesis of cytokines(TNF-α, IL-1β, IL-6 and TGF-β) in head kidney cells was significantly increased in all treatment groups(P<0.05), and the LCS [(23.11±1.31) pg/mL], HK [(991.82±55.86) pg/mL], LCS [(147.98±8.25) pg/mL]/CS[(147.38±7.94) pg/mL] and LCS [(139.56±9.69) pg/mL] treatment groups had the best promotion effect re-spectively. Only the LCS, CS and EPS-2 treatment groups could significantly increase the synthesis ofIL-10(P<0.05), and the LCS [(81.82±3.03) pg/mL] had the best promotion effect on IL-10. Researchshowd that, the different treatment methods of L. Lactis Z-2 all showed a certain immunomodulatory ef-fect on common carp, which provided a theoretical basis for the rational use of potential probiotics and anew solution for the application of lactic acid bacteria to microecological agents.
