关键词：
橙色红曲菌;诱变菌株;固态发酵;桔霉素;大米;高效液相色谱
摘 要：
将橙色红曲菌As3.4384及其诱变菌株接种于大米,30℃静置培养14d,采用HPLC法测定了发酵后的大米中桔霉素含量,结果表明:以SymmetryC18(250×4.6mmI.D.,5μm)色谱柱,乙腈-水(磷酸调pH3.0)(77:23,V/V)为流动相,λex=331nm,λem=500nm为荧光检测波长能较好的对桔霉素进行检测,线性范围为0.01～12.5μg/ml,相关系数为0.999,检测限为0.001μg/ml,As3.4384及其诱变菌株在大米固态发酵物中的桔霉素产量分别为151.40mg/kg和0.218mg/kg。
译 名：
Determination of Citrinin in Fermented Rice of Monascus aurantiaaeus(As3.4384) and Its Mutant Strains by HPLC
作 者：
HUANG Zhi-bing,LI Yan-ping,WANG Yan-hua,XU Yang* (Sino-Germany Joint Research Institute, Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China)
关键词：
Monascus aurantiaaeus;mutant strain;solid-state fermentation;citrinin;rice;HPLC
摘 要：
Monascus aurantiaaeus(As3.4384) and its mutant strains (CSM-11) were inoculated into rice, and were carried static solid-state fermentation for 14 d at 30℃. A reversed-phase high performance liquid chromatographic method has been developed for the determination of citrinin in solid-state fermentation rice. The results showed that the chromatographic separations were carried on a Symmetry C18 column by using acetonitrile-H2O (77:23, V/V, pH3.0) as mobile phases. Fluorophotomeric detection was set at λex=331nm, λem=500nm. The calibration curve was linear in the concentration range of 0.01～12.5μg/ml for citrinin in samples, and the correlation coefficients were 0.999, the limitation of detection (LOD) was 0.001μg/ml. The contents of citrinin for fermentation rice by As3.4384 and CSM-11 were 151.40, 0.218mg/kg respectively.
