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当前位置: 首页 > 文章 > 唐菖蒲电子束诱变作用同功酶及SDS-PAGE分析 北方园艺 2007,2007 (8) 180-182
Position: Home > Articles > Study on Isoenzymes and SDS-PAGE in Induced Gladiolus by Electron Beam Irradiation Northern Horticulture 2007,2007 (8) 180-182

唐菖蒲电子束诱变作用同功酶及SDS-PAGE分析

作  者：
王丹;张志伟;张冬雪;林绍森;匡猛
单  位：
西南科技大学生命科学与工程学院
关键词：
电子束;唐菖蒲;同功酶;SDS-PAGE电泳
摘  要：
为了探讨电子束对唐菖蒲诱变育种的可行性和不同剂量的电子束对唐菖蒲的影响;用能量为3MeV的不同剂量电子束辐照唐菖蒲"江山美人"和"超级"球茎,对不同处理植株的过氧化物酶、酯酶、淀粉酶和过氧化氢酶4种同功酶及SDS-PAGE的变化进行了研究。结果表明:同功酶及SDS-PAGE与对照相比均发生了明显的变化,随着辐照剂量的改变,出现了多种变异形式,变化与形态学变化相一致,但品种间存在较大差异。基于同功酶及SDS-PAGE谱带带型,数据分析使用SPSS11.5,采用单匹配相似系数组内连结分层聚类,分别得到了2品种的聚类树状图,图中显示,"江山美人"被分成了3个组:其中2组分别为200Gy处理材料和240Gy处理材料,另一组包含了小于等于160Gy剂量处理材料及对照;"超级"被分成了3个组:其中2组分别为对照和240Gy处理材料,另一组包含了小于等于200Gy的各处理材料。由聚类分析可以看出"江山美人"比"超级"有更强的电子束耐受性。试验表明,电子束辐照对植株能诱导明显的变异,试验为快中子诱变机理的进一步研究打下基础。
译  名：
Study on Isoenzymes and SDS-PAGE in Induced Gladiolus by Electron Beam Irradiation
作  者：
WANG Dan,ZHANG Zhi-wei,ZHANG Dong-xue,LIN Shao-sen,KUANG Meng(Life Science and Engineering College,Southwest University of Science and Technology,Mianyang,Sichuan 621002,China)
关键词：
Electron beam;Gladiolus;Isozymes;SDS-PAGE electrophoresis
摘  要：
The aim of the experiment is to test the feasibility of electron beam induced mutation on Gladiolus and the effects of various doses of electron beam on Gladiolus.Electron beam radiated on the corm of gladiolus both "chaoji" and"Jiangshanmeiren" with 3 MeV energy and seven doses of 0Gy、40Gy、80Gy、120Gy、160Gy、200Gy、240Gy.SDS-PAGE and the isozymes of POD,CAT,AMY,EST were tested by PAGE technique.The result showed that isozymes of POD,CAT,AMY,EST and SDS-PAGE was obviously changed by electron beam irradiation.The bands of isozymes and SDS-PAGE of different treated materials were studied.Variations of mutagenic effects extent were found among varieties of irradiation dosage,and genetic differentiations are in accord with variations of morphology.But these changes varied greatly among different cultivars.Based on band types of isozymes and SDS-PAGE,the cluster analysis both "Chaoji" and "Jiangshanmeiren" was done respectively and dendrogram generated by within-groups linkage cluster analysis based on Simple matching coefficients of similarity obtained using SPSS software version 11.5.The results indicated that "Jiangshanmeiren" may be divided into three groups:lower dose group(gladiolus radiated at the dose no more than 160 Gy and CK),200 Gy group and 240 Gy group."Chaoji" may be divided into three groups:CK group,lower dose group(gladiolus radiated at the dose no more than 200 Gy)and 240 Gy group.Based on the difference of dendrogram,it was revealed that "Jiangshanmeiren" possessed higher resistance to radiation than "Chaoji",and "Chaoji" was more sensitive to electron beam irradiation than "Jiangshanmeiren".These results showed that electron beam induction was an effective way for Gladiolus breeding.Conclusion these results laid the foundation of electron beam irradiation mutation breeding study.

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