中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines) 农业科学学报 (英文) 2019,18 (12)

Position: Home > Articles > Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines) Journal of Integrative Agriculture 2019,18 (12)

Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)

作者：

Wang Ning;Peng Huan;Liu Shi-ming;Huang Wen-kun;Holgado, Ricardo;Liu-Clarke, Jihong;Peng De-liang

单位：

Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;Norwegian Inst Bioecon Res, NIBIO, Pb 115, NO-1431 As, Norway

关键词：

soybean cyst nematode;lysozyme;Hg-lys1;Hg-lys2;innate defense against bacteria

摘要：

Soybean cyst nematode (SCN, Heterodera glycines (I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes (Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The qRT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles (J2s) were exposed to the Gram-positive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.

相似文章

Assaying the potential of twenty-one legume plants in Medicago truncatula and M-sativa for candidate model plants for investigation the interactions with Heterodera glycines [Kong Ling-an, Wu Du-qing, Huang Wen-kun, Peng Huan, He Wen-ting, Peng De-liang] 农业科学学报(英文版) 2016,15 (3)
Assaying the potential of twenty-one legume plants in Medicago truncatula and M-sativa for candidate model plants for investigation the interactions with Heterodera glycines [Kong Ling-an, Wu Du-qing, Huang Wen-kun, Peng Huan, He Wen-ting, Peng De-liang] 农业科学学报 (英文) 2016,15 (3)
A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant GmSHMT08 [Liu, Zhi, Zhang, Liu-ping, Zhao, Jie, Jian, Jin-zhuo, Peng, Huan, Huang, Wen-kun, Kong, Ling-an, Peng, De-liang, Liu, Shi-ming] 农业科学学报 (英文) 2022,21 (10)
Identification of endophytic Bacillus BHL3501 from wild soybean and its inhibitory effect against soybean cyst nematode (Heterodera glycines Ichinohe). [Yiyong Zhu, Fu-Ming Shi, Jianying Li, Long Ru, Yunpeng Wu, Yunfeng Qia] 植物病虫害研究(英文版) 2011,2 (5)
Comparative Analysis of Gene Expression Profiling Between Resistant and Susceptible Varieties Infected With Soybean Cyst Nematode Race 4 in Glycine max [Li Bin, Sun Jun-ming, Wang Lan, Zhao Rong-juan, Wang Lian-zheng] 农业科学学报 (英文) 2014,13 (12)
Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines) [Ning Wang, Huan Peng, Shiming Liu, Wenkun Huang, Ricardo Holgado, Jihong Liu‐Clarke, Deliang Pen] 农业科学学报(英文版) 2019,18 (12)

PDF下载 PDF预览

导出引用

计量

文章访问数: 14

HTML全文浏览量: 0

PDF下载量: 0

Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)

相似文章

所属期刊

农业科学学报 (英文)

推荐期刊

农业科学学报 (英文)