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Position: Home > Articles > A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant GmSHMT08 Journal of Integrative Agriculture 2022,21 (10)

A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant GmSHMT08

作  者:
Liu, Zhi;Zhang, Liu-ping;Zhao, Jie;Jian, Jin-zhuo;Peng, Huan;Huang, Wen-kun;Kong, Ling-an;Peng, De-liang;Liu, Shi-ming
单  位:
Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;Chinese Acad Agr Sci, Inst Plant Protect, MARA CABI Joint Lab Biosafety, RChina, Beijing 100193, Peoples R China
关键词:
soybean cyst nematode;Heterodera glycines;GmSHMT08;HgHSP70;interaction;resistance
摘  要:
Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production. GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against H. glycines in Peking-type soybeans. However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in H. glycines remains unclear. In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from H. glycines that exhibited interaction with GmSHMT08. This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays. Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08- mediated resistance against SCN H. glycines.

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