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当前位置: 首页 > 文章 > HPLC-MS/MS法同时测定水产品中的诺氟沙星、盐酸小檗碱、盐酸氯苯胍残留 中国兽药杂志 2014,48 (8) 55-60
Position: Home > Articles > Determination of Norfloxacin,Berberine Hydrochloride and Robenidine Hydrochloride Residues in Aquatic Products by High Performance Liquid Chromatography-mass Spectrometry(HPLC-MS/MS) Chinese Journal of Veterinary Drug 2014,48 (8) 55-60

HPLC-MS/MS法同时测定水产品中的诺氟沙星、盐酸小檗碱、盐酸氯苯胍残留

作  者：
汤菊芬;蔡佳;廖建萌;王蓓;秦青英;黄月雄;简纪常
单  位：
广东省水产经济动物病原生物学及流行病学重点实验室;广东海洋大学水产学院;广东省湛江市质量计量监督检测所
关键词：
诺氟沙星;盐酸小檗碱;盐酸氯苯胍;水产品;液质联用
摘  要：
建立了一种同时测定水产品中诺氟沙星、盐酸小檗碱和盐酸氯苯胍残留量的HPLC-MS/MS法。样品经1%甲酸甲醇提取,正己烷脱脂,冷冻、离心后,用高效液相色谱-串联质谱仪,选择反应监测(SRM)、正离子模式进行定性和定量分析。结果表明:诺氟沙星、盐酸小檗碱和盐酸氯苯胍在1~100 ng/mL范围内线性关系良好,线性相关系数≥0.9996,检出限为1 ng/g。诺氟沙星、盐酸小檗碱、盐酸氯苯胍在5、50和100 ng/g三个添加水平下,三种水产品肌肉的平均加标回收率在79.83%~104.06%之间,相对标准偏差在2.97%~9.15%之间。本方法重现性好,灵敏度高,能够满足水产品中诺氟沙星、盐酸小檗碱和盐酸氯苯胍的残留检测和药代动力学的研究需要。
译  名：
Determination of Norfloxacin,Berberine Hydrochloride and Robenidine Hydrochloride Residues in Aquatic Products by High Performance Liquid Chromatography-mass Spectrometry(HPLC-MS/MS)
作  者：
TANG Ju-fen;CAI Jia;LIAO Jian-meng;WANG Bei;QIN Qing-ying;HUANG Yue-xiong;JIAN Ji-chang;Guangdong Ocean University,Fisheries College;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals;Guangdong Zhanjiang Institute of Supervision and Test on Quality and Measure;
关键词：
norfloxacin;;berberine hydrochloride;;robenidine hydrochloride;;aquatic products;;HPLC-MS/MS
摘  要：
An analytical HPLC-MS/MS method was established for determination of norfloxacin(NFLX),berberine hydrochloride(BBH) and robenidine hydrochloride(ROBH) residues in aquatic products. The sampleswere extracted with methanoic acid-methanol(1 ∶ 99,V/V), then defatted with n-hexane. The treated sampleswere analyzed by HPLC-MS/MS after freezed and centrifuged. The analysis was performed with selected reactionmonitoring(SRM) in positive ion mode. The results indicated good linearities in the concentration range from 1~100 ng/mL for NFLX, BBH and ROBH, the correlation coefficient and the detection limit were 0.9997 and1 ng/g, respectively. While the spiked contents of NFLX, BBH and ROBH standard at 5, 50, 100 ng/g, therecoveries and relative standard deviations(RSD) were in range of 79.83% ~104.06% and 2.97% ~9.15%,respectively. This method was suitable for detection and pharmacokinetics study of NFLX, BBH and ROBHresidues in aquatic products.

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