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Position: Home > Articles > A fine-scale genetic analyses of Colletotrichum fructicola strains from Camellia oleifera Acta Phytopathologica Sinica 2016,46 (5) 670-678

小尺度空间果生刺盘孢遗传多样性分析

作  者:
汤铱泠;周国英;李河;刘倩丽;罗娜
关键词:
油茶炭疽病;果生刺盘孢;遗传多样性;多基因
摘  要:
本研究将来自16个油茶品种的247个Colletotrichum fructicola的多基因序列进行遗传多样性分析,结果表明,247个C.fructicola多基因序列有21个单倍型,其中Hap2占总数的54.7%,Hap4占总数的34%,为两个主要的单倍型,来自所有16个油茶品种。基本上每一个油茶品种都存在C.fructicola独特的单倍型。遗传分化系数(Fst)表明不同品种来源菌株种群间存在一定的遗传分化。对所有品种种群进行中性检测及核苷酸不配对分析,发现小尺度空间的果生次盘孢菌经历大规模的种群扩张,群体间存在有效基因流。系统发育分析表明,来自不同品种的菌株散乱地分布在系统发育树中。
译  名:
A fine-scale genetic analyses of Colletotrichum fructicola strains from Camellia oleifera
作  者:
TANG Yi-ling;ZHOU Guo-ying;LI He;LIU Qian-li;LUO Na;SU Song-song;Key Laboratory for Non-w ood Forest Cultivation and Conservation,Ministry of Education;College of Forestry,Central South University of Forestry and Technology;Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests,Central South University of Forestry and Technology;
关键词:
Camellia oleifera anthracnose;;Colletotrichum fructicola;;genetic diversity;;multi-gene
摘  要:
Genetic diversity analysis of 247 Colletotrichum fructicola strains from 16 Camellia oleifera varieties were conducted and 21 haplotypes were identified based on the combined sequences of ITS-CAL-GAPDH.A—mong these,haplotypes 2 and 4 are the two major haplotypes which account for 54.7%and 34%,respectively,and can be identified from all of the 16 C.oleifera varieties.Each variety has its own unique haplotypes of C.fructicola.The coefficient of genetic differentiation(Fst) indicated that there is a certain genetic differentiation among C.fructicola strains derived from different varieties.Neutrality tests and mismatch distribution analysis suggested that there existed large population expansion for fine-scale C.fructicola.The multi-gene phylogenetic analysis showed that strians from different varieties were scattered distribution in the phylogenetic tree.

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