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Position: Home > Articles > Study on Tissue Culture of Eucalyptus dunnii Eucalypt Science & Technology 2012 (4) 20-28

邓恩桉组培技术研究

作  者:
管朝旭;施彬;曾德贤;李基平;陈剑英;何小燕
单  位:
云南省林木种苗工作总站
关键词:
邓恩桉;快速繁殖;黑暗处理
摘  要:
选用不同外植体对邓恩桉组培快繁的初代培养、继代培养、生根培养等各阶段条件进行研究。结果为:以截杆萌发的邓恩桉幼嫩芽条为外植体,采用F+0.5 mg.L-16-BA+0.3mg.L-1IBA培养基,有利于外植体的不定芽诱导以及初期的继代扩大培养,增殖系数达3.68;以改良F+0.25 mg.L-16-BA+0.25-0.5 mg.L-1IBA+20mg.L-1VC+10mg.L-1VB2为培养基,培养初期进行黑暗处理10 d,增殖系数达3.26,小苗高度达1~2.5 cm,适宜用于进一步的生根培养;以DR为邓恩桉生根基本培养基并添加蔗糖40g.L-1+1.0mg.L-1ABT1号生根粉,生根诱导率为84.67%。
译  名:
Study on Tissue Culture of Eucalyptus dunnii
作  者:
GUAN Zhao-xu,SHI Bin,ZENG De-xian,LI Ji-ping,CHEN Jian-ying,HE Xiao-yan(Forestry Seed and Seedling Station of Yunnan Province,Kunming 650215,Yunnan,China)
关键词:
Eucalyptus dunnii;rapid propagation;dark treatment
摘  要:
Different explants from Eucalyptus dunnii were selected to study the optimal conditions for growth at the stages of initial culture,subculture and rooting.The results indicated that adventitious buds from shoot segments of Eucalyptus dunnii could be induced,as well as propagated at the initial subculture stage on a medium of F+0.5 mg·L-1 6-BA +0.3 mg·L-1 IBA,with the multiplication coefficient being 3.68.Improved medium of F+0.25 mg·L-1 6-BA+0.25~0.5 mg·L-1 IBA+20 mg·L-1 VC+10 mg·L-1 VB2 along with dark treatment for 10 days proved appropriate for sprout explants,with the multiplication coefficient and height of seedling being 3.26 and 1~2.5 cm,respectively.Medium of DR supplemented with 40 g·L-1 sugar and 1.0 mg·L-1 1# ABT rooting powder was used for Eucalyptus dunnii rooting culture,with rooting rate of up to 84.7%.

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