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Position: Home > Articles > Establishment and preliminary application of multiplex PCR for rapid detection of Streptococcus suis serotype 2 Animal Husbandry & Veterinary Medicine 2007,39 (9) 4-6

猪链球菌2型多重PCR检测方法的建立及应用

作  者:
覃芳芸;熊毅;盛宇明;白昀;覃伦;朱伟;李华明;潘杰;龙剑明;陈磊;刘棋
单  位:
广西兽医防疫检疫站;广西大学动科院
关键词:
猪链球菌2型;三重PCR;检测
摘  要:
设计3对引物,分别扩增链球菌属特异性gdh、猪链球菌种特异性16S rRNA和猪链球菌2型特异性cps2J等基因,目的片段大小分别为725bp、523bp和387bp。利用合成的3对引物并通过对反应条件与反应体系的优化建立了多重PCR。应用该多重PCR检测了分离到的链球菌1105株,检出猪链球菌667株,猪链球菌2型33株。研究结果表明,该方法特异性高、敏感性强,可广泛应用于猪链球菌病的快速诊断及流行病学调查。
译  名:
Establishment and preliminary application of multiplex PCR for rapid detection of Streptococcus suis serotype 2
作  者:
QIN Fang-yun1,2, XIONG Yi1, SHENG Yu-ming2, BAI Yun2, QIN Lun2, ZHU Wei2,LI Hua-ming1, PAN Jie2, LONG Jian-ming2, CHEN Lei2,LIU Qi1(1.Guangxi General Veterinary Prevention and Quarantine Services, Nanning 530001, China; 2.College of Animal Science and Technology, Guangxi University, Nanning 530005, China)
关键词:
Streptococcus suis type 2; multiplex PCR; detection
摘  要:
Three pairs of primers were designed to amplify three genes, Streptococci genus specific gene (gdh), Streptococci suis species specific gene(16S rDNA)and Streptococci suis type 2 gene(cps2J). The PCR products were 725 bp, 523 bp and 387 bp, respectively. A multiplex PCR was established by optimizing the reaction conditions and used to detect 1 179 samples. The result showed that there were 667 strains of S. suis and 33 strains of S. suis type 2 in 1 105 strains of Streptococcus detected. A sensitive and specific multiplex PCR assay was established for the application in diagnosis and epidemiological survey of S. suis infection.

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