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Position: Home > Articles > A Triple PCR for the Detection of Streptococcus suis Type 2 China Animal Health Inspection 2006,23 (4) 22-24

三重PCR方法检测猪链球菌2型

作  者:
薛素强;范伟兴;洪洁心;黄保续;蔡振鸿;郭霄峰
单  位:
广东出入境检验检疫局动植物监管处;华南农业大学兽医微生物室;国家动物流行病学中心
关键词:
猪链球菌2型;cps2J;epf;mrp;三重PCR;快速检测
摘  要:
根据发表文献,设计合成三对引物,建立三重PCR方法,分别扩增猪链球菌2型cps2J、epf,mrp基因,目标片断大小分别为:230bp、570bp、860bp。对三重PCR方法进行特异性、敏感性、重复性试验,结果显示该三重PCR方法特异性好,敏感性高,重复性好,能快速的检测猪链球菌2型。
译  名:
A Triple PCR for the Detection of Streptococcus suis Type 2
作  者:
Xue SuQiang1), Fan WeiXiang2), HongJiXian3), Huang BaoXu1), Cai ZhenHong2), Guo XiaoFeng1) ( 1) Veterinary Microbiology Laboratory, South China Agricultural University, GuangZhou; 2) China Epizootiology Center, Qingdao 266032; 3) Guangdong Exited Entry Inspection and Quarantive Burean, Guangzhou 510623)
关键词:
Streptococus swi type 2; Triple PCR; Rapid detection
摘  要:
Three pairs of primers were designed and synthesized according to the published literatures and a triple PCR was developed to amplify CPS2J, epf and mrp gene sequences of Streptococus suis Type 2, with the target sequeuces of 230bp, 570bp and 860bp.The triple PCR was tested for its specificity,sensitivity and repeatability.The result showed it could detect as low as 50 germs and with good specificity and repeatability and it could be finished within 7 hours.

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