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Position: Home > Articles > Spectrophotometric Determination of Degree of Substitution of Hydroxypropyl Starch FOOD SCIENCE 2011,32 (22) 201-203

分光光度法测定羟丙基淀粉取代度

作  者:
赵凯;刘丽艳;刘婧婷
单  位:
哈尔滨商业大学食品工程学院黑龙江省高校食品科学与工程重点实验室
关键词:
分光光度法;羟丙基淀粉;取代度
摘  要:
采用分光光度法测定羟丙基淀粉取代度,对测定过程中的影响因素进行分析。结果确定最佳测定条件为测定波长590nm、沸水浴加热3min、冰浴中冷却至15℃后加入茚三酮,在25℃水浴中静置60~100min,在此条件下标准曲线呈现良好的线性关系。经对羟丙基淀粉取代度的实测表明该方法的重复性好。
译  名:
Spectrophotometric Determination of Degree of Substitution of Hydroxypropyl Starch
作  者:
ZHAO Kai,LIU Li-yan,LIU Jing-ting(Key Laboratory of Food Science and Engineering,College of Food Engineering,Harbin University of Commerce, Harbin 150076,China)
关键词:
spectrophotometric method;hydroxypropyl;degree of substitution
摘  要:
A spectrophotometric method to determine the degree of substitution(DS) of hydroxypropyl starch is reported in this paper.Factors that affect the determination were investigated.The optimal sample preparation conditions were heating for 3 min in boiling water bath followed by ninhydrin addition after cooling down to 5 ℃ in ice bath and then reheating in 25 ℃ water bath for 60—100 min.The detection wavelength was selected as 590 nm.Under these conditions,the built standard curve displayed a good linearity.This method allowed the determination of degree of substitution of hydroxypropyl starch with a high repeatability.

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