中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 固定化木瓜蛋白酶水解甲壳胺的研究 中国水产科学 2001,8 (3) 77-80
Position: Home > Articles > Study on depolymerization of chitosan by immobilized papain Journal of Fishery Sciences of China 2001,8 (3) 77-80

固定化木瓜蛋白酶水解甲壳胺的研究

作  者：
王海英;林洪;叶眉;薛长湖
单  位：
青岛海洋大学水产学院
关键词：
木瓜蛋白酶;酶固定化;甲壳胺;水解;低聚糖
摘  要：
木瓜蛋白酶经戊二醛固定于自制的甲壳质上制成固定化木瓜蛋白酶 ,其水解甲壳胺的最佳条件是 :4 5℃ ,pH 4 .0的 1%的甲壳胺溶液 (醋酸缓冲液配置 ) ,酶 /底物比 1∶3.5 (质量比 )。震荡反应 2 4h后 ,用超滤器分离不同分子量的甲壳胺低聚糖产物 ,结果产物中分子量小于 10 0 0 0的甲壳胺低聚糖得率约为 4 5 .5 5 % ,分子量小于 5 0 0 0的约为 34 .0 % ,优于游离木瓜蛋白酶水解甲壳胺的效果。此固定化酶可反复使用至少 10次以上 ,总反应时间达 192h ,提高了酶的使用效率和酶解效果。
译  名：
Study on depolymerization of chitosan by immobilized papain
作  者：
WANG Hai-ying, LIN Hong, YE Mei, XUE Chang-hu (Department of Food Technology & Engineering, Ocean University of Qingdao, Qingdao 266003, China)
关键词：
papain; enzyme immobilization; chitosan; depolymerization; chtooligomers
摘  要：
Glutaric aldehyde as cross linking agent was used to immobilize papain on chitin. The optimum conditions for depolymerizing chitosan by the immobilized papain were as follows: temperature 45℃, pH 4.0, and the weight ratio of immobilized papain to substrate was 1∶3.5. After 24 h's reaction with stirring, the ultrafiltration was used to isolate the chitooligomers into different ranges of molecular weight. The yield rate of chitooligomers with molecular weight below 10 000 was 45.55% to total substrate, and 34% below molecular weight 5 000. The immobilized papain could be used at least 10 times repeatedly, and the total reaction time was more than 192 h, which is longer than 182 h reported previously. When the free papain was used to depolymerize chitosan, the yield rate of chitooligomers with molecular weight below 10 000 was 38.32%. The free papain could be used only once. The result indicated that the depolymerization efficiency of immobilized papain was obviously higher than that of free papain.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46