作 者：
陶菲;童益琴;赵杰;南皓;杨立春;李江浩;葛台明
单 位：
中国地质大学(武汉)湿地演化与生态恢复湖北省重点实验室
关键词：
芝麻(Sesamum indicum L.);SSR标记;FIASCO;遗传多样性
摘 要：
采用FIASCO法构建了芝麻(Sesamum indicum L.)GATA\AAAG\AAAC微卫星富集文库,阳性克隆率62%,其中58%为完美型SSR。利用30个芝麻材料对其中10个扩增稳定、多态性好的SSR位点进行了多样性分析,等位基因数介于4~7之间,平均6.2个;有效等位基因数介于2.18~5.13之间,平均3.73。25个育成品种(系)遗传相似系数平均为0.740 7,遗传距离平均为0.258 9;5个地方品种间的遗传相似系数平均为0.659 3,遗传距离平均值为0.312 8,说明地方品种多样性比育成品种(系)丰富。AMOVA分析表明,品种(系)间变异大于品种(系)内变异,地方品种种内变异大于育成品种(系)。聚类分析表明,一把白与所有育成品种(系)聚为同一类。研究还鉴定出一些遗传差异较大的材料。
译 名：
Development of SSR Markers and Analysis on Genetic Diversity in Sesamum indicum L. Based on FIASCO
作 者：
TAO Fei;TONG Yi-qing;ZHAO Jie;NAN Hao;YANG Li-chun;LI Jiang-hao;GE Tai-ming;Hubei Key Laboratory of Wetland Evolution and Ecological Restoration, China University of Geosciences(Wuhan);School of Environmental Studies, China University of Geosciences(Wuhan);
关键词：
sesame(Sesamum indicum L.);;SSR marker;;FIASCO;;genetic diversity
摘 要：
In this study, 100 clones were randomly selected from the library enriched for GATA\AAAG\AAAC motifs using a modified FIASCO(Fast Isolation by AFLP of Sequences Containing repeats) protocol and sequenced in sesame(Sesamum indicum L.). Sixty-two microsatellite loci, of which 58% were pure repeats, were isolated. 10 highly polymorphic microsatellite markers were obtained, and their polymorphism were assessed among 30 cultivars with 30 individuals per cultivar. The average number of alleles for these loci was 6.2(range 4-7). The effective number of alleles ranged from 2.18 to 5.13 with an average of 3.73. The expected heterozygosity was higher than the observed heterozygosity indicating the inbreeding effect due to breeding. These markers were also used to assess the genetic variation of 30 sesame cultivars. The results showed that the average genetic similarity coefficient and the average genetic distance of 25 breeding varieties were0.7407 and 0.2589 respectively, and the average genetic similarity coefficient and the average genetic distance of 5 landraces were 0.6593 and 0.3128 respectively demonstrating that the genetic diversity of landraces was higher than that of breeding varieties. AMOVA analysis indicated that most genetic variation existed among varieties(strains)(P<0.001). The results also implied that the genetic base of breeding varieties was narrower that that of landraces. Clustering analysis indicated that all30 sesame varieties(strains) were classified into 2 groups. What's more, landrace Yibabai and all breeding varieties(strains)were clustered into 1 group showing that all the tested breeding varieties(strains) had very close genetic relationship. Some varieties with wide genetic variation were identified.
