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当前位置: 首页 > 文章 > 四川松茸内生细菌群落结构与多样性 应用生态学报 2014,25 (11) 3316-3322
Position: Home > Articles > Community structure and diversity of entophytic bacteria in Tricholoma matsutake in Sichuan Province,Southwest China Chinese Journal of Applied Ecology 2014,25 (11) 3316-3322

四川松茸内生细菌群落结构与多样性

作  者：
李强;李小林;黄文丽;熊川;杨阳;杨志荣;郑林用
单  位：
四川省农业科学院土壤肥料研究所;四川省农业科学院生物技术核技术研究所;四川大学生命科学学院
关键词：
松茸;内生细菌;群落结构;PCR-DGGE;促生细菌
摘  要：
为了解松茸的内生细菌群落结构与多样性,以采自四川7个松茸主产区的14个松茸样品为材料,通过变性梯度凝胶电泳(DGGE)分析其内生细菌的群落结构与多样性差异.结果表明:各个产区松茸样品的内生细菌群落结构与多样性存在明显差异.但采自相近地点、相似环境的松茸样品内生细菌群落结构相似性较高;样品的丰度范围为15~25,多样性指数依次为盐源>冕宁>会东>木里>雅江>盐边>小金;条带回收测序构建的系统发育树显示,松茸内生细菌种群丰富,各个产区松茸优势种群存在一定的差异,但假单胞菌属、爱文菌属、芽孢杆菌属在所有样品中均有分布,而且都占据一定的优势地位.产碱杆菌属与鞘氨醇杆菌属在大部分样品中为优势种群;而杜擀氏菌属、赖氨酸芽孢杆菌属在特定样品中为优势种群.表明松茸内生细菌具有丰富的多样性,研究结果可为筛选促进松茸生长的内生菌提供依据.
译  名：
Community structure and diversity of entophytic bacteria in Tricholoma matsutake in Sichuan Province,Southwest China
作  者：
LI Qiang;LI Xiao-lin;HUANG Wen-li;XIONG Chuan;YANG Yang;YANG Zhi-rong;ZHENG Lin-yong;College of Life Sciences,Sichuan University;Soil and Fertilizer Research Institute,Sichuan Academy of Agricultural Sciences;Institute of Biological & Nuclear Technology,Sichuan Academy of Agricultural Sciences;Sichuan Academy of Agricultural Sciences;
关键词：
Tricholoma matsutake;;entophytic bacteria;;community structure;;PCR-DGGE;;growth-promoting bacteria
摘  要：
In order to understand the diversity and community structure of endophytic bacteria in Tricholoma matsutake,14 Tricholoma matsutake samples were collected from 7 main production counties of Xiaojin,Yajiang,Muli,Yanyuan,Yanbian,Huidong and Mianning. The entophytic bacterial community structure and diversity were investigated by PCR denaturing gradient gel electrophoresis( PCR-DGGE). The results showed that the diversity and community structure of endophytic bacteria in T. matsutake varied significantly,while those from the environments alike had high similarity. In addition,each T. matsutake sample contained more than 15 species of endophytes,with that from Yanyuan having the highest endophytes diversity index and abundance,while those from Xiaojin were the lowest. Phylogenetic tree showed that endophytic bacterial species in T.matsutake were abundant. Dominant bacterial population in T. matsutake collected from different places varied,and Pseudomonas,Ewingella and Bacillus were distributed in all samples and retained a certain advantage. Alcaligenes and Sphingobacterium distributed in most samples,while Duganella and Lysinibacillus only in certain ones. This study demonstrated that the endophytic bacteria in T.matsutake were abundant and diversified,which could be in favor of searching the dominant bacteria promoting the growth of T. matsutake.

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