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当前位置: 首页 > 文章 > 高浓度多环芳烃污染土壤的微生物-植物联合修复技术研究 南京农业大学学报 2017 (4) 632-640
Position: Home > Articles > Experimental study on plant-microbial remediation of high concentration PAHs-contaminated soil Journal of Nanjing Agricultural University 2017 (4) 632-640

高浓度多环芳烃污染土壤的微生物-植物联合修复技术研究

作  者：
刘鑫;黄兴如;张晓霞;邱吉国;徐冬青;何健
单  位：
南京农业大学生命科学学院/农业部农业环境微生物重点实验室;中国农业科学院资源环境与区划研究所
关键词：
多环芳烃;土壤;微生物-植物联合修复;紫花苜蓿;盆栽试验;大田试验
摘  要：
[目的]本文旨在研究多环芳烃(PAHs)污染场地微生物-植物联合修复PAHs污染土壤的效果。[方法]利用紫花苜蓿与PAHs降解菌株Rhizobium petrolearium SL-1联合修复土壤中PAHs,设置4个处理:不种苜蓿,不接根瘤菌(CK);不种苜蓿,接根瘤菌(菌);种苜蓿,不接根瘤菌(苜蓿);种苜蓿,接根瘤菌(苜蓿+菌),每个处理设3个重复。盆栽试验土壤取自山东新泰某焦化厂PAHs实地污染土壤,分别于处理20和60 d定期取样;大田试验农田位于河北省唐山市某发电厂附近,分别于处理60和90 d定期取样。测定苜蓿的株高和干质量等生理指标,并利用GC/MS分析土样中的16种PAHs组分降解规律。[结果]盆栽试验中,"苜蓿+菌"处理20和60 d时的苜蓿株高和干质量指标均优于仅种植苜蓿处理;"苜蓿+菌"联合降解PAHs效果明显优于只种植苜蓿或只接菌处理;PAHs不同组分间的降解效果从大到小依次为3环、2环、4环、6环、5环。在大田试验中,60和90 d修复效果同样呈现"苜蓿+菌"联合降解PAHs效果大于只种植苜蓿或只接菌的处理。修复60 d后土壤中低环PAHs的降解率明显高于高环PAHs,PAHs降解效果从大到小依次为2环、3环、4环、5环、6环,但修复90 d后土壤中低环PAHs和高环PAHs的降解率无明显差异。[结论]在PAHs污染土壤或大田试验条件下接种菌株SL-1对紫花苜蓿具有明显的促生作用,并且微生物-植物联合修复比单独微生物或植物修复能更有效地降解PAHs。
译  名：
Experimental study on plant-microbial remediation of high concentration PAHs-contaminated soil
作  者：
LIU Xin;HUANG Xingru;ZHANG Xiaoxia;QIU Jiguo;XU Dongqing;HE Jian;College of Life Sciences/Key Laboratory of Agricultural Environmental Microbiology,Ministry of Agriculture,Nanjing Agricultural University;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences;
关键词：
PAHs;;soil;;plant-microbial remediation;;alfalfa;;pot experiment;;field experiment
摘  要：
[Objectives]The main purpose of this research is to study the bioremediation of polycyclic aromatic hydrocarbons( PAHs)-contaminated soil using plant-microbial remediation systerm. [Methods]The alfalfa and the PAHs-degrading bacterium Rhizobium petrolearium SL-1 were used. One control( CK) was set with noninoculation of alfalfa and strain SL-1. Three treatments were set as follows: inoculation with only alfalfa( alfalfa),inoculation with only strain SL-1( strain),and inoculation of both alfalfa and strain SL-1( alfalfa+strain),respectively. The PAHs-contaminated soil was collected in a coking plant in Xintai,Shandong for the pot experiments. The field experiments were performed on a farm near a power plant in Tangshan,Hebei. The samples were observed at the 20 and 60 d for the pot experiments,and at 60 and 90 d for the field experiments. Physiological indexes,the plant height and dry weight were determined. The content of 16 kinds of PAHs component in soil samples was monitored by GC/MS. [Results]The physiological indexes such as plant height and dry weight of alfalfa in the treatment( alfalfa+strain) were better than those of treatment( alfalfa) at the 20 and 60 d in pot experiment. The PAHs degradation efficiency of the treatment( alfalfa + strain) was significantly higher than those of the treatment only planting alfalfa or inoculating strain. The degradation efficiency for different PAHs ranged from high to low were: 3 ring,2 ring,4 ring,6 ring,5 ring. In the field experiment,the treatment( alfalfa + strain) also showed a better remediation results than treatment( alfalfa) or( strain) at the 60 and 90 d. The degradation rate of low-molecularweight PAHs at 60 d was significantly higher than that of high-molecular-weight PAHs in soil. After 90 d the degradation rate of PAHs was no significantly different among the 16 kinds of PAHs. [Conclusions]Inoculation of strain SL-1 promotes the growth ofalfalfa in the in-situ conditions. Combined remediation of strain SL-1 and alfalfa has better remediation efficiency towards PAHcontaminated soil than single strain SL-1 or alfalfa bioremediation.

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