译 名:
Discussion on the Detection of the Cell Cycle of BHK-21 Cells in Flow Cytometry by DAPI Staining
作 者:
YU Zong-you;YANG Xiao-pu;College of Veterinary Medicine,Gansu Agricultural University;China Agricultural Veterinary Biological Science and Technology Co.Ltd.;
关键词:
DAPI;;Cell cycle;;Flow cytometry
摘 要:
[Objective] The research aimed to lay the foundation for detecting the cells in mass culture. [Method] BHK-21 cells were marked by using 4,6- Diamidine-2 phenylindole( DAPI) staining method to discuss a simple and feasible DNA detection technology. DNA content in each stage of cell cycle was detected. [Result] DAPI staining method was a simple,reliable and feasible DNA detecting method.[Conclusion]The cell cycle could be accurately detected by DAPI labeling method.