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Position: Home > Articles > Discussion on the Detection of the Cell Cycle of BHK-21 Cells in Flow Cytometry by DAPI Staining Journal of Anhui Agricultural Sciences 2015 (23) 149-150

DAPI染色法在流式细胞术中检测BHK-21细胞周期的探讨

作  者:
俞宗佑;杨孝朴
单  位:
中农威特生物科技股份有限公司;甘肃农业大学动物医学院
关键词:
DAPI染色法;细胞周期;流式细胞技术
摘  要:
[目的]为规模化细胞培养的检测奠定基础。[方法]采用DAPI标记BHK-21细胞,探索简便易行的DNA检测技术,检测细胞周期中各期的DNA含量。[结果]DAPI标记法是一种可靠的DNA检测方法,且简单易行。[结论]利用DAPI标记法能够准确进行细胞周期检测。
译  名:
Discussion on the Detection of the Cell Cycle of BHK-21 Cells in Flow Cytometry by DAPI Staining
作  者:
YU Zong-you;YANG Xiao-pu;College of Veterinary Medicine,Gansu Agricultural University;China Agricultural Veterinary Biological Science and Technology Co.Ltd.;
关键词:
DAPI;;Cell cycle;;Flow cytometry
摘  要:
[Objective] The research aimed to lay the foundation for detecting the cells in mass culture. [Method] BHK-21 cells were marked by using 4,6- Diamidine-2 phenylindole( DAPI) staining method to discuss a simple and feasible DNA detection technology. DNA content in each stage of cell cycle was detected. [Result] DAPI staining method was a simple,reliable and feasible DNA detecting method.[Conclusion]The cell cycle could be accurately detected by DAPI labeling method.

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