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当前位置: 首页 > 文章 > 山楂叶有效成分提取工艺优化及抗氧化活性研究 核农学报 2015,29 (8) 1547-1558
Position: Home > Articles > Optimization of Extraction Technology of Effective Components From Hawthorn Leaves and Assessment of Antioxidant Activity Journal of Nuclear Agricultural Sciences 2015,29 (8) 1547-1558

山楂叶有效成分提取工艺优化及抗氧化活性研究

作  者：
弓威;顾丰颖;贺凡;王锋
单  位：
中国农业科学院农产品加工研究所/农业部农产品加工综合重点实验室
关键词：
山楂叶;总黄酮;超声波;响应面设计;抗氧化
摘  要：
本试验以山楂叶为研究对象,选取乙醇体积分数、料液比、提取时间、提取温度为自变量,总黄酮得率为响应值,进行四因素三水平的Box-Benhnken试验。利用响应面分析法优化山楂叶总黄酮超声提取工艺,并对山楂叶总黄酮清除DPPH自由基、ABTS自由基能力及其还原力进行探讨。结果表明,超声波辅助乙醇提取山楂叶总黄酮的最佳工艺条件为乙醇体积分数70%,料液比1∶35,提取时间39min,提取温度72℃,在此工艺条件下总黄酮得率预测值为29.198 mg·g-1,实测值为28.991 mg·g-1,与理论预测值的相对误差仅为0.71%。山楂叶总黄酮清除DPPH自由基和ABTS自由基的半抑制浓度(IC50)分别为718.96μg·m L-1和64.43μg·m L-1,而且对Fe3+具有一定的还原能力。试验结果表明,山楂叶总黄酮具有一定的抗氧化活性。本研究为山楂叶功能成分的进一步开发利用提供了科学依据和技术参考。
译  名：
Optimization of Extraction Technology of Effective Components From Hawthorn Leaves and Assessment of Antioxidant Activity
作  者：
GONG Wei;GU Fengying;HE Fan;WANG Feng;Comprehensive Key Laboratory of Agro-Products Processing,Ministry of Agriculture/Institute of Agro-products Processing Science and Technology,Chinese Academy of Agricultural Sciences;
关键词：
hawthorn leaves,total flavonoids,ultrasound-assisted,response surface methodology,antioxidant activity
摘  要：
The objective of the study is to optimize ultrasound-assisted ethanol extraction of flavonoids from hawthorn leaves and investigate the antioxidant activity of total flavonoids in vitro. Hawthorn leaves were used in this paper. The response surface methodology was used to investigate the effects of processing parameters, including ethanol concentration,solid-liquid ratio,extraction time and temperature. The yield of flavonoids was chosen as response value,and mathematical model was established by implementing four factors and three levels. The antioxidant activity of total flavonoids was evaluated by DPPH,ABTS radical scavenging and reducing power assays. The results indicated that the best extraction technology was ethanol concentration of 70%,solid-liquid ratio of 1 ∶ 35,extraction time of 39 min and extraction temperature of 72℃. The actual flavonoids yield was 28. 991 mg·g- 1,which showed a relative error of0. 71% compared with the predicted value( 29. 198 mg·g- 1). The concentrations for 50% inhibition( IC50) of DPPH radical scavenging activity of total flavonoids was 718. 96 μg·m L- 1and IC50 value was 64. 43μg·m L- 1in ABTS radical scavenging assays. The iron ions reduction results showed that total flavonoids had certain reducing power. These results indicated that total flavonoids of hawthorn leaves have good antioxidant activity in vitro. The research can provide a scientific theory basis for further development and technical reference of hawthorn leaves.

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