单 位：
云南省林业和草原科学院;西南林业大学材料与化学工程学院
关键词：
流苏香竹;竹叶黄酮;响应面优化;抗氧化;亚硝化反应
摘 要：
为了提高竹叶黄酮的得率，以流苏香竹叶为研究对象，采用响应面法优化流苏香竹竹叶黄酮超声协同乙醇提取的工艺，并测定流苏香竹叶提取物抗氧化、清除亚硝化物以及阻断亚硝化反应的能力。结果表明，响应面优化超声协同乙醇提取的最佳工艺条件为：乙醇浓度为66.5%,料液比1︰30,超声功率311 W,流苏香竹叶黄酮的得率为37.146 mg/g。生物活性研究表明，流苏香竹叶提取物在一定浓度范围内对羟基自由基和超氧阴离子的清除能力强于L-抗坏血酸(Vc),DPPH自由基清除能力稍弱。对亚硝化物的清除率可达到52.59%,亚硝化反应的阻断率可达到74.75%。相关性分析表明，黄酮类化合物是流苏香竹叶中主要的抗氧化剂，亚硝化物的清除及亚硝化反应的阻断呈高度正相关。该研究发现流苏香竹竹叶黄酮作为多靶点抗癌药物或保健产品的潜力很大，可促进流苏香竹叶高附加值产品的开发。
译 名：
Ultrasonic-assisted Ethanol Extraction of Flavones from Chimonocalamus fimbriatus Leaves and Their Removal and Blocking Effects on Nitrosation Reaction
作 者：
LI Aoxiang;TAN Yanfang;FENG Wu;College of Materials and Chemical, Southwest Forestry University;Yunnan Academy of Forestry and Grassland;
关键词：
Chimonocalamus fimbriatus;;bamboo leaf flavonoid;;response surface optimization;;oxidation resistance;;N-nitrosation
摘 要：
In order to improve the yield of flavonoids from Chimonocalamus fimbriatus leaves, the response surface method was used to optimize the ultrasonic-assisted ethanol extraction of flavonoids from C.fimbriatus leaves.The antioxidant, nitrosation-removing and nitrosation-blocking abilities of the extracts were measured.The results showed that the optimal conditions of response surface optimization were ethanol volume fraction of 66.5%,solid-liquid ratio of 1︰30,ultrasonic power of 311 W,and the yield of flavonoids from bamboo leaves was 37.146 mg/g.The biological activity study showed that the scavenging ability of bamboo leaf extract on hydroxyl radical and superoxide anion was stronger than L-ascorbic acid(Vc),and the scavenging ability of DPPH free radical was slightly weaker in certain concentration range.The removal rate of nitrite can reach 52.59 %,and the blocking rate of nitrosation can reach 74.75 %.The correlation analysis showed that flavonoids were the main antioxidants in C.fimbriatus leaves, and the removal of nitrosation and blocking of nitrosation reaction were highly correlated.This study found that the flavonoids of phyllostachys argifolia have great potential as multi-target anticancer drugs or health care products, and can promote the development of high value-added products of C.fimbriatus.
