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Position: Home > Articles > Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate FOOD SCIENCE 2005,26 (9)

枯草杆菌纤溶酶高产菌株的物理化学诱变

作  者:
吕凤霞;陆兆新;别小妹;梁思宇
单  位:
南京农业大学食品科技学院
关键词:
枯草杆菌;纤溶酶;辐射;硫酸二乙酯;突变株
摘  要:
以枯草杆菌FM-S2作为出发菌株,经γ-射线和2.5%硫酸二乙酯复合诱变,结合牛奶蛋白平板从2341株存活菌中初筛得到200株诱变菌株,经摇瓶测定纤溶酶活性进行复筛得到7株生产性能较出发菌株显著提高的突变株,它们的纤溶酶活性超过2000UK.U/ml。其中突变株γ-DES36纤溶酶活性最高达到2719.89±242.51UK.U/ml,同时该菌株遗传性能稳定。
译  名:
Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate
作  者:
Lü Feng-xia,LU Zhao-xi*,BIE Xiao-mei,LIANG Si-yu (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
Bacillus Subtilis;fibrinolysin;radiation;DES;mutants
摘  要:
Bacillus Subtilis FM-S2, as parental strain, was mutagenized withγ-ray and DES. 200 mutants were selected by the isolation on the milk protein plates from 2341 survival.mutants. Seven of mutants over 2000UK.U/ml were obtained by measuring the fibrinolysin activity. It was found that γ-DES36 strain had the highest fibrinolysin activity, which reached 2719.89±242.51UK.U/ml, and that it was stable for 10 generations.

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