中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究 安徽农业科学 2012,40 (8) 4609-4611
Position: Home > Articles > Proliferation of Avian Influenza Virus H9 Subtype in MDCK Cells in Different Culture Media Journal of Anhui Agricultural Sciences 2012,40 (8) 4609-4611

不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究

作  者：
张建伟;史爱华;沈佳;景小冬;章振华;李林;姜北宇
单  位：
北京市农林科学院畜牧兽医研究所
关键词：
培养基;H9亚型流感病毒;MDCK细胞;增殖效果
摘  要：
[目的]比较3种培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果。[方法]采用DMEM+10%血清、低血清培养基(MEM-MD-611)、无血清培养基(SFE4Mega)3种培养基制备MDCK细胞单层,分别接种不同稀释度的H9亚型禽流感病毒。然后,分别加入含10μg/ml胰蛋白酶的3种培养基作为维持液,培养病毒,每隔24 h观察其细胞病变,并测定上清HA滴度。[结果]在培养72～96 h时,无血清培养基病毒液的HA滴度高于低血清培养基,而低血清培养基则高于含血清培养基。[结论]3种培养基均可用于制备禽流感病毒抗原,其中无血清培养基、低血清培养基更适用于流感病毒抗原的制备。
译  名：
Proliferation of Avian Influenza Virus H9 Subtype in MDCK Cells in Different Culture Media
作  者：
ZHANG Jian-wei et al(Institute of Animal Husbandry and veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097)
关键词：
Culture medium;Avian influenza virus H9 subtype;MDCK cell;Proliferation
摘  要：
[Objective]To screen the best culture media for proliferation of avian influenza virus(AIV) H9 subtype in MDCK cells.[Method]The MDCK monolayer cells were respectively cultured in DMEM containing 10%(V/V) newborn calf serum(NBS),low-serum culture medium(MEM-MD-611) and serum-free medium(SFE4Mega) and then inoculated with different dilutions of AIV H9 subtype.These three kinds of media containing 10 μg/ml trypsin were also used for maintenance culture of AIV.Every 24 h,cytopathic changes were observed and the HA titer of the culture supernatant was also determined.[Result]After 72-96 h of culture,the HA titers of the culture supernatant derived from the serum-free medium,low-serum culture medium and serum containing medium were in a decreasing manner.[Conclusion]The DMEM containing 10%(V/V) newborn calf serum(NBS),low-serum culture medium(MEM-MD-611) and serum-free medium(SFE4Mega) can be used for proliferation of AIV,and the latter two may be more suitable to prepare AIV culture.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46